小鼠急性胰腺炎中Shh在胰腺局部和远隔器官的表达变化  

Expression of Shh in different tissues in acute pancreatitis

作　　者：赖坤 罗澜 王芳 何雪梅[2] 周翔宇 

机构地区：[1]西南医科大学附属医院血管甲状腺外科,四川泸州646000 [2]西南医科大学附属医院医学实验中心,四川泸州646000

出　　处：《西南医科大学学报》2017年第1期26-30,共5页Journal of Southwest Medical University

基　　金：国家自然科学基金(81100272)

摘　　要：目的:探讨Sonic Hedgehog(Shh)信号在小鼠急性胰腺炎中胰腺及远隔组织的表达及意义。方法:腹腔注射雨蛙素诱导急性胰腺炎模型,检测淀粉酶水平,用RT-qPCR、Western blotting、免疫组织化学方法检测Shh在胰腺、以及胰腺远隔组织肝、肾、小肠、肺组织的表达与分布。结果:雨蛙素腹腔注射后的实验组各组织(胰腺、肝、小肠、肺、肾)Shh表达均较对照组升高。结论:在急性胰腺炎过程中,全身炎症反应综合征可能会引起胰腺及远隔组织的损伤,而Shh信号可能参与了急性胰腺炎过程中远隔组织损伤和修复过程。Objective：To investigate tissue distribution of Shh in acute pancreatitis in mice. Methods： Acute pancreatitis model was induced by intraperitoneal injection of Caerulein, and the expression of Shh in pancreas, liver, kidney, small intestine, lung tissue were analyzed by RT-qPCR and Western blotting and immunohistoc-hemistry（IHC）. Results： Compared with the control group, the expression of Shh in all organs analyzed was higher in the exprerimental group. Conclusion： In acute pancreatitis, inflammation may affect local and distant organs and Shh signaling pathway may be involved in injury and repair of distal organs in acute pancreatitis.

关 键 词：SHH 远隔组织 急性胰腺炎 

分 类 号：R576.045[医药卫生—消化系统]