MiR-195对BEL-7402/5-FU细胞5-氟尿嘧啶耐药性的影响  

Effects of MicroRNA-195 on 5-FU Resistance in BEL-7402/5-FU Cells

作　　者：杨晓燕[1] 向琼[1] 殷杰[1] 虞佳[1] 甘润良[1] 雷小勇[1] 

机构地区：[1]南华大学药物药理研究所,湖南衡阳421001

出　　处：《中南医学科学杂志》2017年第1期32-34,47,共4页Medical Science Journal of Central South China

基　　金：国家自然科学基金资助(81372579);湖南省卫生厅医药卫生资助科研项目(B2014-048);湖南省教育厅一般课题(13C832)

摘　　要：目的探讨miR-195对BEL-7402/5-FU细胞5-氟尿嘧啶药物敏感性的影响。方法采用qRTPCR检测BEL-7402细胞与BEL-7402/5-FU细胞中miR-195的表达水平。将miR-195质粒载体瞬时转染至BEL-7402/5-FU细胞;MTT检测细胞药物敏感性的改变。结果相比于BEL-7402细胞,miR-195在BEL-7402/5-FU细胞中表达水平下调;miR-195转染组的细胞增殖抑制率较miRNA阴性对照组及未转染组明显增高。结论MiR-195能够增加BEL-7402/5-FU细胞对5-氟尿嘧啶的药物敏感性,抑制细胞增殖。Objective This study investigated the effect of miR-195 on 5-FU resistance in BEL-7402 / 5-FU Cells.Methods qRT-PCR analyzed expression of miR-195 between BEL-7402 and BEL-7402 / 5-FU cells.MiR-195 was transiently transfected into BEL-7402 / 5-FU cells.Drug sensitivity of the cells to 5-FU was analyzed by MTT. Results The expression of miR-195 was downregulated in BEL-7402 / 5-FU cells compared to parental BEL-7402 cells.MTT results showed that miR-195 transfected cells had a higher cell inhibition rate than untreated cells or negative control. Conclusions MiR-195 could sensitize BEL-7402 / 5-FU cells to 5-FU and inhibited cell proliferation.

关 键 词：MiR-195 BEL-7402/5-FU细胞 5-氟尿嘧啶 耐药性 

分 类 号：R735.7[医药卫生—肿瘤]