IL-18对胰腺星状细胞及其趋化因子CX3CL1表达的影响  

The influence of IL-18 on pancreatic stellate cells and CX3CL1 expression

作　　者：王茜[1] 陈浩宇[1] 陈洁[1] 

机构地区：[1]第二军医大学长海医院消化内科,上海200433

出　　处：《中华胰腺病杂志》2017年第1期31-34,共4页Chinese Journal of Pancreatology

基　　金：国家自然基会青年科学基会项目（81300352）

摘　　要：目的观察IL-18对胰腺星状细胞E-钙黏素（E-cadherin）、α-平滑肌肌动蛋白（α-SMA）及趋化因子CX3CL1表达水平的影响。方法人胰腺星状细胞（PSCs）株HPaSteC常规培养、传代，应用5、25、50、100 μg/L IL-18干预72 h，以未干预细胞为对照组。收集各组细胞，采用RT-PCR和蛋白质印迹法检测细胞E-cadherin、α-SMA、CX3CL1 mRNA及蛋白表达量。结果对照组及5、25、50、100 μg/L IL-18干预组PSCs的E-cadherin mRNA表达量分别为1.03±0.17、0.77±0.15、0.89±0.12、0.54±0.11、0.46±0.06；α-SMA mRNA表达量为1.03±0.19、0.85±0.14、1.33±0.22、1.60±0.14、1.94±0.09；CX3CL1 mRNA表达量为1.01±0.08、0.88±0.25、0.86±0.17、1.58±0.26、1.83±0.13。干预组E-cadherin mRNA的表达下调，α-SMA及CX3CL1mRNA表达上调，其中100 μg/L干预组与对照组的差异均有统计学意义（P〈0.05或〈0.01）。对照组及5、25、50、100 μg/L IL-18干预组PSCs的E-cadherin蛋白表达量分别为1.00±0.14、1.14±0.04、1.14±0.07、0.85±0.08、0.80±0.06；α-SMA蛋白表达量为1.00±0.02、0.77±0.07、1.29±0.02、1.59±0.07、1.70±0.02；CX3CL1蛋白表达量分别为1.00±0.05、1.03±0.05、1.37±0.06、1.46±0.18、1.45±0.12。干预组E-cadherin蛋白表达下调，但各组间的差异无统计学意义；α-SMA蛋白表达上调，25、50、100 μg/L IL-18干预组与对照组的差异有统计学意义（P值均〈0.01）；CX3CL1蛋白表达上调，其中100 μg/L干预组与对照组的差异有统计学意义（P〈0.05）。结论IL-18能激活人PSCs并上调PSCs趋化因子CX3CL1的表达。Objective To evaluate the effect of IL-18 on the expression of E-cadherin, α-SMA and CX3CLI in pancreatic stellate cells（PSCs）. Methods The human PSC line HPaSteC was routinely cultured and passaged. Five, 25, 50 and 100 p.g/L IL-18 was used to treat PSCs for 72 h and the untreated PSCs were used as control. Treated and untreated cells were both collected, and RT-PCR and Western blot were used to detect mRNA and protein expression of E-cadherin, α-SMA and CX3CL1 respectively. Results The mRNA expressions of E-cadherin in control group and 5, 25, 50 and 100 μg/L IL-18 treated group were 1.03 ± 0.17, 0.77 ±0. 15, 0.89 ± 0. 12, 0. 54 ± 0. 11 and 0.46 ± 0.06. The mRNA expression of μ-SMA were 1.03 ± 0. 19, 0. 85 ± 0. 14, 1.33 ± 0. 22, 1.60 ± 0. 14 and 1.94 ± 0.09; The mRNA expression of CX3CL1 were 1.01 ± 0.08, 0.88 ± 0.25, 0.86 ± 0. 17, 1.58 ± 0.26 and 1.83 ± 0.13. The mRNA expression of E-cadherin in IL-18 treated group were down-regulated , while the mRNA expression of α-SMA and CX3CL1 were upregulated, and the differences between control and IL-18 100 μg/L treated group were statistically significant （P 〈0.05 or 〈0.01 ）. The protein expression of E-cadherin in control group and 5, 25, 50 and 100 μg/L IL-I 8 treated group were 1.00 ± 0.14,1.14 ± 0.04, 1.14 ± 0.07, 0.85 ± 0.08 and 0.80 ± 0.06. The protein expression of α-SMA were 1. 00 ± 0. 02, 0.77 ± 0.07, 1.29 ± 0.02, 1.59 ± 0.07 and 1.70 ± 0.02 ; The protein expression of CX3CL1 were 1.00 ± 0.05, 1.03 ± 0.05, 1.37 ± 0.06, 1.46 ± 0.18 and 1.45 ± 0.12. The protein expression of E-cadherin was down-regulated but no significant differences were observed among different groups. The protein expression of α-SMA was up-regulated and the differences between control group and 25, 50 and 100 μg/L IL-18 treated groups were statistically significant （all P〈0.01 ）. The protein expression of CX3CL1 was up-regulated and the differences between control group and 100 ng/ml IL-18 treated group were statistically significant

关 键 词：胰腺 星形细胞 胰腺炎 慢性 趋化因子CX3CL1 白细胞介素18 

分 类 号：R576[医药卫生—消化系统]