细胞凋亡通路在小鼠酒精性胰腺炎发展过程中的作用  

Cell apoptosis pathway in the development of alcoholic pancreatitis in mice

作　　者：郭敏[1] 余鹏飞[1] 白槟[1] 邱兆岩 王谦[1] 赵青川[2] 李树钧[2] 

机构地区：[1]第四军医大学西京消化病医院肿瘤生物学国家重点实验室,西安710032 [2]第四军医大学西京消化病医院消化ICU,西安710032

出　　处：《中华胰腺病杂志》2017年第1期35-39,共5页Chinese Journal of Pancreatology

基　　金：国家自然科学基金（81370564）;陕西省社会发展攻关计划项目（2014SF2-11）

摘　　要：摘要 目的探讨细胞凋亡通路在酒精性胰腺炎中的作用。方法将C57BL/J小鼠随机分为对照组（NC）、酒精组（AC）、急性胰腺炎组（AP）和酒精性胰腺炎组（AAP）。饮酒精方案为10%乙醇喂养2 d，15%乙醇喂养5 d，20%乙醇喂养直到13周。采用腹腔注射雨蛙素50 μg/kg体重，连续7次，每次间隔1 h的方法制备AP模型。取血检测血清淀粉酶、脂肪酶活性；取部分胰腺称湿、干重，计算胰腺组织水含量；取部分胰腺组织常规行病理学检查；采用蛋白质印迹法检测胰腺组织凋亡相关蛋白caspase3、caspase8表达，TUNEL法检测胰腺组织的细胞凋亡。结果NC组、AC组、AP组、AAP组小鼠血清淀粉酶活性分别为（3 630±259）、（3 196±187）、（35 955±4 607）、（53 607±3 848）U/L，脂肪酶活性为（502±41）、（745±42）、（7 346±665）、（12 764±2 544）U/L，胰腺水含量为（70.2±3.1）%、（69.6±2.0）%、（78.2±1.5）%、（85.0±3.0）%，胰腺组织caspase 3表达量为1.017±0.078、1.287±0.097、2.018±0.078、0.244±0.024，caspase 8表达量为0.829±0.010、0.599±0.074、1.270±0.080、0.145±0.015，凋亡细胞数为1、6、214、97个/10倍视野，胰腺病理评分为0、0、（7.0±0.4）、（12.8±0.3）分。AP组的血清淀粉酶、脂肪酶、胰腺水含量、胰腺病理评分均显著高于NC组，AAP组又均显著高于AP组，差异有统计学意义（P〈0.05或〈0.01）；AP组的caspase3、caspase 8表达量、细胞凋亡数均显著高于NC组，而AAP组的这些指标均显著低于AP组，且caspase3、caspase8表达量均显著低于NC组，差异有统计学意义（P值均〈0.05）。结论长期饮用酒精可加重胰腺炎的严重程度，其机制可能是通过抑制细胞凋亡，促进腺泡细胞坏死所致。Objective To explore the role of cell apoptosis pathway in alcoholic pancreatitis. Methods C57BL/J mice were divided into control group（NC） and Alcohol group （ AC）, Acute pancreatitis group （AP） and Alcoholic acute pancreatitis group （AAP）. Alcohol treatment was 10% w/v ethanol feeding for 2 d, 15% w/v ethanol for 5 d, and then 20% w/v ethanol until 13 weeks. AP model was established by the intraperitoneal injection of 50μg eaerulein/kg body weight once an hour for a total of 7 times. Blood samples were collected for detecting serum amylase and lipase activity. Part pancreatic tissue was collected and the wet and dry weight were both measured to calculate the water content. The routine pathological examination of the pancreatic tissues were conducted. The expression of apoptosis associated protein caspase3 and caspase8 was determined by Western blot. And cell apoptosis was determined using TUNNEL method. Results The level of serum amylase in NC group, AC group, AP group and AAP group were （ 3 630 ± 259 ） , （ 3 196 ± 187 ）, （ 35 955 ± 4607 ） and （ 53 607 ± 3 848 ） U/L ; the level of serum lipase were （ 502 ± 41 ）, （ 745 ± 42 ）, （ 7 346 ± 665） and （ 12 764 ± 2 544 ） U/L; the water content were （ 70.2 ± 3.1 ） %, （ 69.6 ± 2.0 ） %, （ 78.2 ± 1.5 ） % and（85.0 ± 3.0） % and （ 12.75 ± 0.25 ） ; the expression of caspase3 were （ 1. 017 ± 0. 0784 ）, （ 1. 287 ± 0.097）, （ 178 ± 0.07785） and （ 0.2443 ± 0.0243 ） ; the expression of easpase8 were （ 0. 8289 ±0. 0096 ）, （0.5985 ± 0. 0735 ）, （ 1.27± 0.08 ） and （0. 145 ± 0. 015 ） ; the number of apoptotic cells were 1, 6, 214, 97/10 high power field. The pathological score of pancreas injure in NC group, AC group, AP group and AAP group were 0, 0, （7 ± 0.4 ） and （ 12.8 ± 0.3 ）, respectively. Serum amylase, lipase, water content and pathological scores in AP group were obviously higher than those in NC group （P 〈0.05 ） , which in AAP group were also

关 键 词：胰腺炎 酒精性 乙醇 细胞凋亡 小鼠 

分 类 号：R576[医药卫生—消化系统]