中药复方制剂治疗桃源鸡大肠杆菌病的试验  

Experiment on Treating Tao-Yuan Chicken Colibacillosis by Compound Chinese Herbal Medicine

作　　者：张保平[1] 李娜[2] 

机构地区：[1]常德职业技术学院农林工程系,湖南常德415000 [2]湖南文理学院动物学湖南省高校重点实验室,湖南常德415000

出　　处：《天津农业科学》2017年第3期17-20,共4页Tianjin Agricultural Sciences

基　　金：常德职业技术学院青年项目(ZY0908);湖南省教育厅科学研究项目(12C0974);湖南省常德市科技计划项目(2012CK03;2011CK01);国家星火计划项目(2015GA770010)

摘　　要：为比较5组中药复方制剂在体外抑菌作用的效果,筛选对常德地区桃源鸡大肠杆菌抑菌作用较好的组方,按中医药配伍原则,选用15种单味中药配伍出5组复方制剂。采用常规水煎煮法制备水提物,分别用平板打孔法、试管二倍梯度稀释法及平板接种法对临床分离的鸡大肠杆菌进行药物敏感性和最小抑菌浓度测定。以对临床分离的大肠杆菌株敏感性较高的头孢曲松作对照,采用自拟中药复方制剂对人工感染的桃源鸡大肠杆菌病进行治疗试验和预防保护性试验,并对其疗效进行评价。结果表明,中药复方制剂I(I紫地榆、石榴皮、黄连)和复方制剂IV(五倍子、乌梅、黄芩)水提液对大肠杆菌体外抑菌作用较好,其最小抑菌浓度分别是0.016,0.008 mg·mL^(-1);中药复方制剂II(I黄柏、白芍、诃子)和复方制剂V(白头翁、马齿苋、茯苓)水提液抑菌作用次之,最小抑菌浓度均是0.063 mg·mL^(-1);复方制剂(I唐菖、大黄、金银花)抑菌作用最小,其抑菌浓度为0.500 mg·mL^(-1),其菌落数随药物浓度升高呈降低趋势。中药复方制剂II和头孢曲松都可以有效控制人工感染大肠杆菌后桃源鸡的临床症状,明显降低鸡只死亡率,中药复方制剂II和头孢曲松对大肠杆菌病治疗率分别是80.0%和86.6%,都高于感染组;中药复方制剂II预防大肠杆菌病的保护率是83.3%,与头孢曲松一致。The fifteen Chinese herbal medicines were Gladiolus gandavensis, Radixet rhizome, Rhei palmati, Lonicera japonica, Gera- nium strictipes, Pericarpium punicae Granati, Rhizoma coptidis, Phellodendron amurense Rupr., Paeonia sterniana Fletcher in Journ., Fructus chebulae, Galla chinensis, Fructus mume, Radix scutellariae Baicalensis, Radix pulsatillae, Herba portulacae Oleraceae, Glabrous greenbrier Rhizome. The bacteriostatic activities of 5 groups compound chinese herbal medicine prescriptions against entero- toxigenic Escherichia coli were observed by Plate punching method and serial dilution test. Compound chinese herbal medicine pre- scription was selfmade according to Chinese veterinary theory. With ceftriaxone highly sensitive to the isolated strain of Escherichia colias control, the cure and preventing protective test were made on colibacillosis in chicken infected artificially to evaluate its cura- tive effect on Tao-Yuan chicken ColibaciUosis. Escherichia coli was highly sensitive to the compound chinese herbal medicine pre- scriptions II and IV, The minimal inhibitory concentration （MIC） were 0.016, 0.008 mg.mL^- 1. Moderately sensitive to the medicine prescriptions III and V, both MIC were 0.063 rag. mL^-1. But prescriptions I was lowly sensitive effect, MIC was 0.500 rag. mL^-1. The Chinese herbal medicine and its medicinal formula had better treatment effect, low residue and low drug resistance. Both Chinese herbal compound prescription and ceftriaxone could control the clinical symptoms of chicken infected artificially by Escherichia coli effectively and decrease the death rate significantly. The cure protective rate of Chinese herbal compound prescription and ceftriaxone to Escherichia coli were 80.0 % and 86.6 %, respectively, greatly higher than that in infection control group. Chinese herbal com- pound prescription had better protective effect on preventing colibacillosis in chicken with the protective rate of 83.3 %, which was the same with that of ceftriaxone.

关 键 词：中药复方制剂 桃源鸡 大肠杆菌病 疗效 

分 类 号：S858.31[农业科学—临床兽医学]