原癌基因c-met在肝脏离断门静脉分支结扎大鼠术后剩余肝中的表达  

Expression of proto-oncogene c-met in the future liver remnant after liver partition and portal vein ligation in rats

作　　者：安华松 李向农 杨军 丁亮 杨勇 庄磊 韩亚东 汪源 

机构地区：[1]徐州医科大学研究生学院,江苏徐州221002 [2]徐州医科大学附属医院普外科,江苏徐州221002

出　　处：《中国肿瘤外科杂志》2017年第1期13-16,共4页Chinese Journal of Surgical Oncology

摘　　要：目的观察大鼠肝脏离断+门静脉分支结扎术后剩余肝组织中c-met基因和蛋白的表达及其在术后剩余肝快速再生中的作用。方法将雄性SD大鼠96只应用计算机产生随机化数字完全随机分为肝脏离断+门静脉分支结扎术组、单纯门静脉分支结扎术组和假手术组。检测术后24 h、72 h、7 d和10 d时各组大鼠剩余肝组织中c-met基因和蛋白的表达。结果肝脏离断+门静脉分支结扎术组术后剩余肝组织中c-met mRNA和蛋白的表达均增加,并于7(10.09±1.41)d达到峰值,明显高于单纯门静脉分支结扎术组的(3.49±0.94)(P<0.05)。结论肝脏离断+门静脉分支结扎术后大鼠剩余肝组织中原癌基因c-met表达明显升高。Objective To observe the expression of c-met gene and protein in the future liver remnant （FLR） after liver partition and portal vein ligation （ LP ＋ PVL） and its role in the rapid regeneration of the rem- nant liver after operation. Methods A total of 96 male SD rats were randomly divided into 3 groups with 32 rats in each group including the liver partition ＋ portal vein ligation group （ the LP ＋ PVL group） , the portal vein ligation group （the PVL group） and the sham operation group （the Sham group）. The expressions of c-met gene and protein in the future liver remnant （FLR） were measured after 24 h, 72 h, 7 d and l0 d in each group. Results After operation, the expression of c-met mRNA and protein increased, and reached the peak value at 7 （10.09 ± 1.41 ） d, and was significantly higher than that in the PVL group （3.49±0. 94） d （P 〈 0.05 ）. Conclusions The expression of e-met was significantly increased in the future liver remnant after liver partition and portal vein ligation.

关 键 词：肝脏离断术 门静脉分支结扎术 C-MET 肝再生 大鼠 

分 类 号：R735.7[医药卫生—肿瘤]