不同浓度核黄素-蓝光交联对兔眼巩膜组织生物力学的影响  

Investigation of the biomechanics of blue-light cross-linking on rabbit scleral tissue with different concentrations of riboflavin

作　　者：邹迎[1] 张淼[1] 张丰菊[1] 

机构地区：[1]首都医科大学附属北京同仁医院北京同仁眼科中心北京市眼科学与视觉科学重点实验室,100730

出　　处：《中华眼视光学与视觉科学杂志》2017年第1期9-13,共5页Chinese Journal Of Optometry Ophthalmology And Visual Science

基　　金：国家自然科学基金（81070763,81570877）;北京市教育委员会科技计划重点项目（KZ20130025027）;北京市卫生系统高层次卫生技术人才基金（2013-2-023）

摘　　要：目的通过使用不同浓度核黄素联合蓝光照射对兔巩膜组织行胶原交联，评估交联后的巩膜生物力学强度．探讨蓝光-核黄素交联法较合理的核黄素浓度。方法实验研究。选取成年健康新西兰白兔共10只（20眼）。根据随机数字法分为2组，每组5只。均选取右眼为实验眼，左眼为对照眼，2组分别应用0．1％（Ⅰ组）和0．5％（Ⅱ组）核黄素溶液联合460nm蓝光进行巩膜胶原交联，术后2个月处死动物．取巩膜标本进行单轴拉伸试验，检测应力-应变指标，即杨氏模量、极限应力。采用配对t检验及独立样本t检验对数据进行分析。结果兔眼巩膜的应力-应变曲线呈非线性变化，符合σ=Aexp（Bxe）指数方程．2组实验眼杨氏模量均较对照眼高，差异具有统计学意义（Ⅰ组：t=19．78，P〈0．01；Ⅱ组：t=7．82，P〈0．01）；类似的，实验眼极限应力也有明显提高，差异具有统计学意义（Ⅰ组：t=4．90，P〈0．05；Ⅱ组：t=4．30，P〈0．05）；Ⅰ组实验眼杨氏模量较Ⅱ组高，差异具有统计学意义（t=2．52，P〈0．05）；类似的，Ⅰ组实验眼极限应力也较Ⅱ组高，差异具有统计学意义（t=3．03，P〈0．05）。结论蓝光一核黄素胶原交联法可有效增强兔眼巩膜的生物力学强度，0．1％为核黄素一蓝光交联法较合理的核黄素浓度，能更大限度地增强交联术后巩膜的生物力学强度。Objective This study had two objectives： To evaluate the efficiency of collagen cross-linking （CXL） in rabbit sclera induced by different concentrations of riboflavin and blue light. To find the optimal concentration of riboflavin in scleral cross-linking. Methods In this experimental study, scleral stress-strain measurementswere performed to evaluate the biomechanical efficiency of collagen cross-linking using riboflavin and blue light （460 nm）. Ten New Zealand rabbits were randomly divided into Group Ⅰ and Ⅱ. In Group Ⅰ, a 0.1% riboflavin solution was used for the CXL induced by blue light. In Group Ⅱ, a 0.5% riboflavin solution was used. In each group, the right eye was defined as the experimental eye and the left eye was the control. Biomechanical measurements of scleral strips were performed 2 months after CXL using a microcomputer-controlled biomaterial testing device. The focus was on Young＇s modulus calculated as σ=Aexp（Bxe） and maximum stress. The data were analyzed by paired sample t-test and two independent sample t-test. Results Scleral specimens from both groups exhibited nonlinear stress-strain behavior with an initially low Young＇s modulus that increased gradually under higher stress. It was derived using the equation σ=Aexp（Bxe）. The Young＇s modulus （Group Ⅰ ： t=19.78, P〈0.01; Group Ⅱ： t=7.82, P〈0.01） and maximum stress （Group Ⅰ ： t=4.90, P〈0.05; Group Ⅱ： t=4.30, P〈0.05） of the sclera increased after CXL. In addition, the scleral strips in Group Ⅰ were stiffer than in Group Ⅱ （Young＇s modulus： t=2.52, P〈0.05; Maximum stress： t=3.03, P〈0.05）. Conclusion CXL induced by riboflavin/blue light can significantly enhance the biomechanical strength of rabbit sclera. The 0.1% riboflavin solution had significantly better biomechanical behavior than the 0.5% riboflavin in CXL.

关 键 词：胶原交联 巩膜 核黄素 生物力学 蓝光 

分 类 号：R772.3[医药卫生—眼科]