百脉根小GTPaseROPs基因反转座子插入突变体的分离和鉴定  

Isolation and identification of small GTPase ROPs retrotransposons insertion mutants in Lotus japonicus

作　　者：李碧璇[1] 张晴[1] 朱辉[1] 张忠明[1] 

机构地区：[1]华中农业大学生命科学技术学院/农业微生物学国家重点实验室,武汉430070

出　　处：《华中农业大学学报》2017年第2期45-52,共8页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(31170224;31370278);农业微生物学国家重点实验室"815"项目(AMLKF200804)

摘　　要：为研究ROP(Rho-related GTPase of plants,ROP)在豆科植物共生固氮过程中的功能和作用机制,从百脉根逆转录转座子LORE1插入突变体库(http://www.kazusa.or.jp/lotus)中搜索到6个ROP相关基因突变体,通过分离和鉴定,得到不同突变体的M1代和M2代纯合体种子,对M2代进行表型鉴定及统计分析。结果表明:在早期共生表型的鉴定中,突变体与野生型植株相比,rop-like1植株的根瘤原基数明显下降,rop-like4植株的侵入线数和侵入线密度也明显下降;但仅rop-like1植株在接种14d后的结瘤数明显减少。Six ROPs related gene mutants were found through screening mutants library of the Lotus retrotransposons 1insertion（http：//users-mb.au.dk/pmgrp）.Different homozygote M1 and M2generation seeds were obtained by the isolation and PCR identification.Results of identification and statistical analysis of M2 generation symbiotic phenotype showed that the root nodules primordium numbers per plants of rop-like1 homozygote were significantly decreased compared with those of wild type.Both the numbers and density of infection threads per plant in rop-like4 homozygote were also significantly decreased.The nodule numbers of rop-like1 homozygote decreased significantly in comparison with those of wild type after 14 days inoculation.It will provide evidence for further studying ROPs function and regulatory mechanism in rhizobium infection and the process and development of nodules organs.

关 键 词：百脉根 LORE1 反转录转座子 ROPS 

分 类 号：Q939.9[生物学—微生物学]