云南哈尼梯田当前栽培水稻ALK基因的遗传多样性及与稻米糊化温度的关联分析  

Genetic Diversity of ALK Gene and Its Association with Grain Gelatinization Temperature in Currently Cultivated Rice Landraces from Hani's Terraced Fields in Yunnan Province

作　　者：李闯[1,2] 刘承晨 张昌泉 朱霁晖 徐小颖[2] 赵福伟[3] 黄绍文[4] 金银根[2] 刘巧泉 

机构地区：[1]扬州大学江苏省作物遗传生理国家重点实验室培育建设点/粮食作物现代产业技术协同创新中心,江苏扬州225009 [2]扬州大学生物科学与技术学院,江苏扬州225009 [3]环境保护部南京环境科学研究所,江苏南京210042 [4]云南红河学院,云南蒙自661100

出　　处：《作物学报》2017年第3期343-353,共11页Acta Agronomica Sinica

基　　金：国家重点基础研究发展计划项目(2013CBA01402);国家自然科学基金项目(31561143008;31300324);江苏省高校自然科学研究面上项目(13KJB180028)资助~~

摘　　要：云南是世界公认的亚洲栽培稻遗传多样性和起源中心之一。利用51对SSR分子标记对云南哈尼梯田当前栽培的111份水稻材料进行多态性检测,并分析其群体结构。聚类分析表明,供试材料主要分为偏粳类群(group I)及偏籼类群(group II)两大类群,其中以偏籼类群居多,占83%,这两类群并未完全按地理来源聚类。所选材料稻米的糊化温度变异较广,可以分为低(<66℃)、中低(66~70℃)、中高(70~74℃)和高(>74℃)4类。通过基因测序,分析了稻米糊化温度控制基因ALK的序列多样性,发现其可以分为10种单倍型。关联分析结果表明,所选水稻样品稻米的糊化温度主要由ALK基因3个SNP位点组合控制。其中,A-GC和G-TT两种组合主要控制低(<66℃)和中低(66~70℃)糊化温度,G-GC组合主要控制中高(70~74℃)和高(>74℃)糊化温度。SNP3402 T类型与高、中高糊化温度有关。绝大多数偏籼类品种的ALK基因含有G-GC组合,因此都倾向于高糊化温度。上述研究结果为水稻分子育种和种质资源保护研究提供了一定的参考价值。Yunnan province is one of the centers for genetic diversity of cultivated rice in Asia.A total of 111 rice landraces were collected from Hani＇s rice terraces of Yunnan,and their population structure was analyzed by using 51 SSR markers.The 111 rice landraces were divided into japonica group（group I） and indica group（group II）;however,each of the two groups was not completely clustered according to its geographical origin.Most collected landraces,accounting for 83%,belonged to indica group.Gelatinization temperature（GT） is an important parameter affecting rice cooking and eating quality.The GT of rice flour was measured,which was widely different among the collected rice varieties,showing four classes in very low（66°C）,low（66？70°C）,high（70？74°C） and very high（74°C） GT.Meanwhile,ALK gene,also known as SSSIIa and controlling GT,was sequenced and compared,showing ten haploid types of the ALK gene among tested samples.Association analysis,revealed that GT is mainly controlled by the combination of three SNPs of ALK gene.Among them,the A-GC or G-TT combination type controls the very low and low GT,while the combination of G-GC controls high and very high GT.SNP3402 type T is associated with high and very high GT.It was also noticed that most of the varieties from indica group tended to contain the G-GC mutation and thus showed a relative high GT compared with those from the japonica group.These results are meaningful for rice molecular breeding and germplasm resources protection.

关 键 词：水稻 ALK基因 遗传多样性 哈尼梯田 糊化温度 

分 类 号：S511[农业科学—作物学]