Apo-E基因敲除小鼠中MMP-9的表达研究（英文）  

In Addition to Studies in Mice Expressing MMP-9 Knockout of Apoe

作　　者：伍韵贤[1,2] 曾昭华 杜一鹏 苏子焯 

机构地区：[1]广州医科大学附属第一医院心血管内科,广州510120 [2]广州市海珠区瑞宝街社区卫生服务中心,广州510288

出　　处：《血栓与止血学》2017年第2期181-185,189,共6页Chinese Journal of Thrombosis and Hemostasis

基　　金：supported by Science and Technology Planning Project of Guangdong Province(No.2013B021800291);Science and Technology Program of Guangzhou Municipal Government(No.2014Y2-00186);National Natural Science Foundation of China(No.81170280)

摘　　要：目的通过观察Apo-E(-/-)小鼠主动脉根部血管MMP-9的表达情况,进一步了解MMP-9与动脉粥样硬化斑块形成过程的相关性。方法本实验采用免疫组织化学和逆转录聚合酶链技术对MMP-9在基因敲出小鼠动脉中的表达进行研究。结果正常小鼠冠状动脉中MMP-9有微量表达,动脉粥样硬化斑块中检测到MMP-9 mRNA和MMP-9蛋白的表达明显增高。结论 MMP-9可能参与小鼠动脉粥样硬化病变过程,并在小鼠主动脉根部血管壁斑块中表达增高。Objective To investigate the expression of matrix metalloproteinase-9 （MMP-9）in the aor- tic root of Apo-E（-/-） mice and to explore the correlation between MMP-9 and atherosclerotic plaque forma- tion. Methods The expression of MMP-9 in the arteries of knockout mice was studied by immunohistochernis- try and reverse transcription polymerase chain reaction （RT-PCR）. Results The expression of MMP-9 and MMP-9 was significantly increased in the coronary arteries of normal mice. The expression of MMP-9 mRNA and MMP-9 protein was significantly increased in atherosclerotic plaques. Conclusion MMP-9 may be in- volved in the process of atherosclerosis in mice, and the expression of MMP-9 in vascular wall plaque of aortic root in mice is increased.

关 键 词：MMP-9 Apo-E(-/-)小鼠 动脉粥样硬化 

分 类 号：R36[医药卫生—病理学]