罹患重度牙周炎磨牙拔除后应用去蛋白牛骨基质与可吸收胶原膜进行位点保存的组织学研究  

Histomorphometric evaluation of ridge preservation after molar tooth extraction

作　　者：詹雅琳 胡文杰[1] 徐涛[1] 甄敏[1] 路瑞芳[1] 

机构地区：[1]北京大学口腔医学院·口腔医院,牙周科口腔数字化医疗技术和材料国家工程实验室,口腔数字医学北京市重点实验室,北京100081

出　　处：《北京大学学报（医学版）》2017年第1期169-175,共7页Journal of Peking University:Health Sciences

基　　金：首都卫生发展科研专项基金(2011-4025-04);首都临床特色应用研究与成果推广基金(Z161100000516042)资助~~

摘　　要：目的:观察罹患重度牙周炎磨牙拔牙位点同期植入Bio-Oss~并覆盖Bio-Gide~胶原膜愈合6个月后组织形态学特点和成骨效果。方法:6名研究对象共6颗罹患牙周病变磨牙拔除后即刻在拔牙窝内植入Bio-Oss~颗粒覆盖Bio-Gide~胶原膜。术后6个月,翻瓣再进入,在位点保存区取一块直径2.8 mm、长度6.0 mm的圆柱状骨组织,制作甲苯胺蓝染色的硬组织切片,进行组织学检查和骨组织形态计量学分析。结果:骨组织形态计量学结果显示平均11.54%(0~28.40%)新生骨形成和35.04%(13.92%~50.87%)Bio-Oss~颗粒残留,其中20.13%(0~48.50%)Bio-Oss~颗粒与新生骨组织直接接触,79.87%(51.50%~100.00%)颗粒被结缔组织包裹;上颌位点新生骨和Bio-Oss~颗粒百分比均较下颌位点少。结论:组织学检查发现罹患重度牙周炎磨牙拔牙窝内植入Bio-Oss~并覆盖Bio-Gide~胶原膜愈合6个月后有新骨形成,仍有较多Bio-Oss~颗粒残留。Objective： To evaluate bone formation in human extraction sockets with absorbed surrounding walls augmented with Bio-Oss~and Bio-Gide ~after a 6-month healing period by histologic and histomorphometric analyses. Methods： Six fresh molar tooth extraction sockets in 6 patients who required periodontally compromised moral tooth extraction were included in this study. The six fresh extraction sockets were grafted with Bio-Oss ~particle covered with Bio-Gide ~. The 2. 8 mm × 6. 0 mm cylindric bone specimens were taken from the graft sites with aid of stent 6 months after the surgery. Histologic and histomorphometric analyses were performed. Results： The histological results showed Bio-Oss~particles were easily distinguished from the newly formed bone,small amounts of new bone were formed among the Bio-Oss~particles,large amounts of connective tissue were found. Intimate contact between the newly formed bone and the small part of Bio-Oss ~particles was present. All the biopsy cylinders measurement demonstrated a high inter-individual variability in the percentage of the bone,connective tissues and BioOss~particles. The new bone occupied 11. 54%（ 0-28. 40%） of the total area; the connective tissues were 53. 42%（ 34. 08%-74. 59%） and the Bio-Oss~particles were 35. 04%（ 13. 92%-50. 87%）. The percentage of the particles,which were in contact with bone tissues,amounted to 20. 13%（ 0-48. 50%）. Conclusion： Sites grafted with Bio-Oss~particles covered with Bio-Gide ~were comprised of connective tissues and small amounts of newly formed bone surrounding the graft particles.

关 键 词：牙周炎 引导组织再生 牙周 牙槽嵴增高术 生物相容性材料 组织学 

分 类 号：R781.42[医药卫生—口腔医学]