ABCB1和ABCC2基因多态性与中国汉族儿童抗结核药物致肝毒性易感性的相关性研究  

Association Between Polymorphisms of ABCB1 and ABCC2 Genes and the Susceptibility to Anti-tuberculosis Drug-induced Hepatotoxicity in Chinese Han Children

作　　者：李颖佳[1] 汤海京[2] 綦辉[1] 申晨[1] 孙琳[1] 李洁琼[1] 徐放[1] 焦伟伟[1] 杨旭[2] 申阿东[1] LI Ying-jia TANG Hai-jing QI Hui SHEN Chen SUN Lin LI Jie-qiong XU Fang JIAO Wei-wei YANG Xu SHEN A-dong(MOE Key Laboratory of Major Diseases in Children, National Key Discipline of Pediatrics（ Capital Medical University）, National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute,Beijing Children＇s Hospital, Capital Medical University, Beijing 100045, China)

机构地区：[1]首都医科大学附属北京儿童医院、北京市儿科研究所,儿科学国家重点学科,儿科重大疾病研究教育部重点实验室,国家呼吸系统疾病临床医学研究中心,儿童呼吸道感染性疾病研究北京市重点实验室,北京100045 [2]北京理工大学软件学院,北京100081

出　　处：《标记免疫分析与临床》2017年第2期121-126,共6页Labeled Immunoassays and Clinical Medicine

基　　金：国家自然科学基金应急管理项目(81441067);国家自然科学基金面上项目(81571950);北京市科委行业定额经费资助(2014-bjsekyjs-1);北京市科技新星计划交叉学科合作课题(xxjc2001611);2015年北京市留学人员科技活动择优资助项目

摘　　要：目的探讨ABCB1和ABCC2基因多态性与中国汉族儿童抗结核药物致肝毒性(anti-tuberculosis drug-induced hepatotoxicity,ATDH)易感性的相关性。方法在中国汉族结核病患儿中,采用病例对照研究,利用高通量的Mass ARRAY平台,对于ABCB1和ABCC2基因的16个标签单核苷酸多态性(single nucleotide polymorphisms,SNPs)位点展开基因分型,并采用logistic回归分析以上SNP位点等位基因和基因型频率在ATDH病例组和ATDH对照组中的分布差异。结果本研究共纳入41例ATDH病例以及189例对照。ABCB1和ABCC2基因SNP位点的等位基因以及基因型在两组间频率分布差异均无统计学意义(P>0.05)。SNP位点分别按照显性遗传模型和隐性遗传模型分析,各位点基因型在两组间频率分布差异均无统计学意义(P>0.05)。结论 ABCB1和ABCC2基因多态性可能与中国汉族儿童ATDH的发生并无相关性。Objective To investigate the association between polymorphisms of ABCB1 and ABCC2 genes and anti-tuberculosis drug-induced hepatotoxicity（ATDH） in Chinese Han children. Methods A case-control study was performed in Chinese Han pediatric patients with tuberculosis. Mass ARRAY was used for genotyping in 16 tag single nucleotide polymorphisms（SNPs） of ABCB1 and ABCC2 genes. Then,logistic regression was used to analyze differences of allele and genotype distributions of SNP polymorphisms between the case and control groups. Results Totally 41 incident ATDH cases and 189 ATDH-free controls were included in this study. No significant differences were found in the allele and genotype distributions of all SNPs in ABCB1 and ABCC2 genes between the case and control groups（ P〈0. 05）. And there were also no significant differences observed in the genotype distributions of all SNPs in the dominant and recessive heredity models（ P〈0. 05）. Conclusion SNPs of ABCB1 and ABCC2 genes were not associated with ATDH in Chinese Han children.

关 键 词：抗结核药物致肝毒性 ABCB1 ABCC2 单核苷酸多态性 汉族儿童 

分 类 号：R725.7[医药卫生—儿科] R529.9[医药卫生—临床医学]