二次培养对大龙骨藻总脂、EPA及PUFA含量的影响  

Effect of the secondary culture on contents of total lipids,EPA and PUFA of Tropidoneis maxima

作　　者：丁玉惠[1] 蒋霞敏[1] 张泽凌[1] 韩庆喜[1] 梁晶晶[1] 

机构地区：[1]宁波大学海洋学院,浙江宁波315211

出　　处：《中国油脂》2017年第2期107-112,共6页China Oils and Fats

基　　金：国家海洋公益项目(201305022)

摘　　要：为探究二次培养(低温和高光照强度胁迫)对大龙骨藻(Tropidoneis maxima)总脂、EPA及PUFA含量的影响,将大龙骨藻置于适宜条件(温度25℃,光照强度36μmol/(m2·s),盐度25)下培养10 d后,采用单因子试验,在低温(10℃)或高光照强度(80μmol/(m^2·s))条件下进行二次培养,并进一步进行低温、高光照强度二因子三水平二次培养试验。结果表明:二次培养最佳时间为3 d;低温10℃有利于EPA和PUFA积累,分别可达(28.48±0.47)%和(43.28±1.06)%;光照强度80μmol/(m^2·s)有利于总脂、EPA和PUFA积累,分别可达(43.19±1.29)%、(27.11±0.25)%和(41.78±0.22)%;在低温10℃、高光照强度80μmol/(m^2·s)交互作用下更有利于总脂、EPA、PUFA积累,分别可达(42.99±2.51)%、(32.83±0.58)%和(46.43±0.22)%。综上所述,二次培养方式有利于藻生长和油脂积累达到较高水平,为提高微藻产油量提供了一种新的培养方法,值得深入研究。To explore the effects of the secondary culture （ low temperature and high light intensity stress） on the contents of total lipids, EPA and PUFA of Tropidoneis maxima, Tropidoneis maxima was cultured for 10 d under the conditions of temperature 25 ℃, light intensity 36 μmol/（ m^2·s） and salinity 25, then was secondarily cultured under low temperature （10 ℃ ） or high light intensity （80 μmol/（ m^2 · s） ） by single factor experiment, and the two - factor three - level secondary culture experiment of low temper- ature and high light intensity was further conducted. The results showed that the optimal secondary culture time was 3 d;low temperature of 10℃ was beneficial to the accumulation of EPA and PUFA, and they could reach （28.48 ± 0.47） % and （43.28 ±1.06） %, respectively; the light intensity of 80 gmol/（ m^2 · s ） also had a positive effect on the accumulation of total lipids, EPA and PUFA, reaching（43.19 ± 1.29）%, （ 27.11 ± 0.25 ） % and （41.78 ± 0.22 ） %, respectively. And it was more beneficial to the accumulation of total lipids, EPA and PUFA under the interaction between low temperature 10 ℃ and light intensity 80 p, mol/（m^2 · s）, and their contents were （42.99±2.51）%, （32.83±0.58）% and （46.43± 0.22） % , respectively. Above all, the secondary culture was favorable to obtain higher levels of growth and lipid accumulation of algae. It provided a novel culture method for improving lipid production of al- gae, and was worthy for further study.

关 键 词：大龙骨藻 二次培养 总脂 EPA PUFA 

分 类 号：Q946[生物学—植物学]