Pretreated quercetin protects gerbil hippocampal CA1 pyramidal neurons from transient cerebral ischemic injury by increasing the expression of antioxidant enzymes  

作　　者：Bai Hui Chen Joon Ha Park Ji Hyeon Ahn Jeong Hwi Cho In Hye Kim Jae Chul Lee Moo-Ho Won Choong-Hyun Lee In Koo Hwang Jong-Dai Kim Il Jun Kang Jun Hwi Cho Bich Na Shin Yang Hee Kim Yun Lyul Lee Seung Min Park 

机构地区：[1]Department of Histology and Embryology,Institute of Neuroscience,Wenzhou Medical University,Wenzhou,Zhejiang Province,China [2]Department of Biomedical Science and Research Institute for Bioscience and Biotechnology,Hallym University,Chuncheon,South Korea [3]Department of Neurobiology,School of Medicine,Kangwon National University,Chuncheon,South Korea [4]Department of Pharmacy,College of Pharmacy,Dankook University,Cheonan,South Korea [5]Department of Anatomy and Cell Biology,College of Veterinary Medicine,and Research Institute for Veterinary Science,Seoul National University,Seoul,South Korea [6]Division of Food Biotechnology,School of Biotechnology,Kangwon National University,Chuncheon,South Korea [7]Department of Food Science and Nutrition,Hallym University,Chuncheon,South Korea [8]Department of Emergency Medicine,School of Medicine,Kangwon National University,Chuncheon,South Korea [9]Department of Physiology,College of Medicine,and Institute of Neurodegeneration and Neuroregeneration,Hallym University,Chuncheon,South Korea [10]Department of Surgery,School of Medicine,Kangwon National University,Chuncheon,South Korea [11]Department of Emergency Medicine,Sacred Heart Hospital,College of Medicine,Hallym University,Anyang,South Korea

出　　处：《Neural Regeneration Research》2017年第2期220-227,共8页中国神经再生研究（英文版）

基　　金：supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education(NRF-2015R1D1A1A01059728);Hallym University Research Fund 2014(HURF-2014-25)

摘　　要：Quercetin（QE; 3,5,7,3′,4′-pentahydroxyflavone）, a well-known flavonoid, has been shown to prevent against neurodegenerative disorders and ischemic insults. However, few studies are reported regarding the neuroprotective mechanisms of QE after ischemic insults. Therefore, in this study, we investigated the effects of QE on ischemic injury and the expression of antioxidant enzymes in the hippocampal CA1 region of gerbils subjected to 5 minutes of transient cerebral ischemia. QE was pre-treated once daily for 15 days before ischemia. Pretreatment with QE protected hippocampal CA1 pyramidal neurons from ischemic injury, which was confirmed by neuronal nuclear antigen immunohistochemistry and Fluoro-Jade B histofluorescence staining. In addition, pretreatment with QE significantly increased the expression levels of endogenous antioxidant enzymes Cu/Zn superoxide dismutase, Mn superoxide dismutase, catalase and glutathione peroxidase in the hippocampal CA1 pyramidal neurons of animals with ischemic injury. These findings demonstrate that pretreated QE displayed strong neuroprotective effects against transient cerebral ischemia by increasing the expression of antioxidant enzymes.Quercetin（QE; 3,5,7,3′,4′-pentahydroxyflavone）, a well-known flavonoid, has been shown to prevent against neurodegenerative disorders and ischemic insults. However, few studies are reported regarding the neuroprotective mechanisms of QE after ischemic insults. Therefore, in this study, we investigated the effects of QE on ischemic injury and the expression of antioxidant enzymes in the hippocampal CA1 region of gerbils subjected to 5 minutes of transient cerebral ischemia. QE was pre-treated once daily for 15 days before ischemia. Pretreatment with QE protected hippocampal CA1 pyramidal neurons from ischemic injury, which was confirmed by neuronal nuclear antigen immunohistochemistry and Fluoro-Jade B histofluorescence staining. In addition, pretreatment with QE significantly increased the expression levels of endogenous antioxidant enzymes Cu/Zn superoxide dismutase, Mn superoxide dismutase, catalase and glutathione peroxidase in the hippocampal CA1 pyramidal neurons of animals with ischemic injury. These findings demonstrate that pretreated QE displayed strong neuroprotective effects against transient cerebral ischemia by increasing the expression of antioxidant enzymes.

关 键 词：nerve regeneration flavonoids transient cerebral ischemia Cu/Zn superoxide dismutase catalase Mn superoxide dismutase glutathione peroxidase neural regeneration 

分 类 号：R743.3[医药卫生—神经病学与精神病学]