人胚胎干细胞抑制人肝癌细胞系SK-Hep1增殖并促进凋亡  

Human embryonic stem cells inhibit proliferation and promote apoptosis of human hepatoma cell line SK-Hep1

作　　者：张婷[1,2] 霍本念 刘洁[1,2] 刘梦楠[1] 冯涛[1,2] 

机构地区：[1]重庆医科大学分子医学与肿瘤研究中心,重庆400016 [2]重庆医科大学重庆市生物化学与分子药理学重点实验室,重庆400016

出　　处：《基础医学与临床》2017年第3期325-329,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(81071770)

摘　　要：目的探究在人胚胎干细胞hESCs与人肝癌细胞系SK-Hep1共培养微环境中,hESCs对SK-Hep1人肝癌细胞增殖、侵袭、迁移和凋亡的影响。方法建立hESCs与SK-Hep1人肝癌细胞系的非接触式共培养体系,将与hESCs共培养的SK-Hep1细胞作为实验组,单独培养的SK-Hep1细胞作为对照组。MTT法检测SK-Hep1细胞的增殖能力;Transwell小室法检测SK-Hep1的侵袭与迁移能力;Hoechst33258染色后观察共培养后SK-Hep1细胞核的变化;流式细胞术检测SK-Hep1细胞凋亡率。结果与hESCs共培养后,SK-Hep1细胞的增殖能力受到抑制,随着时间增加,抑制效果越显著(P<0.05);细胞侵袭、迁移穿过Transwell小室的数量显著减少(P<0.05);发生核固缩、变形和浓染的SK-Hep1细胞较对照组增多;细胞凋亡比率较对照组显著增加(P<0.05)。结论人胚胎干细胞对SKHep1人肝癌细胞系有抑制作用。Objective To explore effects of human embryonic stem cells（ hESCs） on proliferation,invasion and migration of SK-Hep1 human hepatoma cells in the co-culture of micro environmen of hESCs and SK-Hep1.Methods Single cultured SK-Hep1 cells were served as control group while SK-Hep1 which non-contact co-cultured with hESCs was regarded as experimental group. The proliferation ability of SK-Hep1 was measured by MTT method; invasion and migration ability of SK-Hep1 cells were detected by Transwell chamber method; the nucleus variation and cell apoptosis of SK-Hep1 were detected by Hoechst33258 chromosome and flow cytometry.Results The proliferation of SK-Hep1 cells in the experimental group was obviously inhibited as compared with control group（ P 0. 05）; the number of SK-Hep1 cells which passed through the Transwell chambers were significantly reduced as compared with control group in invasion and migration experiment（ P 0. 05）; more nucleus pycnosis and deformation appeared in experimental group than that in control group. And apoptosis rate of SK- Hep1 cells in the experimental group was significantly higher than that of in the control group（ P 0. 05）.Conclusions Human embryonic stem cells have inhibitory effect on human hepatoma cell line SK-Hep1.

关 键 词：胚胎干细胞 肝癌细胞 抑制作用 

分 类 号：R735.7[医药卫生—肿瘤]