乙酰化α-微管蛋白感受细胞外基质变化调控乳腺癌侵袭转移  

Acetylated a-tubulin sense the change of extracellular matrix to regulate the invasion and metastasis of breast cancer

作　　者：邱霓[1] 何玉芳[1] 李洪胜[1] 

机构地区：[1]广州医科大学附属肿瘤医院乳腺外科,广州恶性肿瘤治疗转化医学重点实验室,广州510095

出　　处：《中国医师杂志》2017年第2期186-190,共5页Journal of Chinese Physician

基　　金：广州市教育局项目（1201630281）

摘　　要：目的揭示乙酰化α-微管蛋白作为压力感受器感受细胞外基质变化对乳腺癌侵袭转移的影响。方法在乳腺癌临床标本和不同分子亚型乳腺癌细胞株中检测乙酰化α-微管蛋白的表达情况及与侵袭转移的关系。通过化学方法干预乙酰化α-微管蛋白的表达，检测在不同基质硬度条件下，细胞形态、侵袭转移能力的变化。结果乙酰化α-微管蛋白在基底型乳腺癌组织和细胞株中的表达最高，而在Luminal B型的乳腺癌组织和乳腺癌细胞株中表达最低。乙酰化α-微管蛋白的表达量与乳腺癌是否发生转移呈正相关。在软基质培养条件下，细胞的极化程度下降，多呈圆形或椭圆形的形态；乙酰化α-微管蛋白表达越低，极性程度越差，伴随侵袭转移能力越弱。结论乙酰化α-微管蛋白作为压力感受器，感受肿瘤发生过程中细胞外基质硬化，促进乳腺癌侵袭转移。Objective To reveal acetylated a-tubulin acts as pressure sensors, sensing the changes in extracellular matrix to impact on the invasion and metastasis of breast cancer. Methods The acetylated alpha mierotubule protein expressions were detected, and its relationship with invasion and metastasis in breast cancer clinical specimens and different molecular subtypes of breast cancer cell lines were analyzed. The expression of acetylated a-tubulin was interfered through chemical methods, and cell morphology and the change of the invasion and metastasis ability were detected under the condition of different matrix hardness. Results The expression of acetylated a-tubulin was highest in basal-like breast cancer tissue and cell lines, and was lowest expressed in the luminal B breast cancer tissue and the breast cancer cell lines. The expression of aeetylated a-microtubule was positively correlated with the occurrence of breast cancer. Under the condition of soft substrate cultivation, cell polarization was declined, becoming the circular or oval shape. The acetylated a-tubulin caused reduction in cell polarity, accompanied with less invasion and metastasis ability. Conclusions The acetylated atubulin acts as pressure sensors, sensing the sclerosis of extraeellular matrix in the process of tumorigenesis, promoting invasion and metastasis of breast cancer.

关 键 词：微管蛋白/代谢 细胞外基质/代谢 乳腺肿瘤 肿瘤侵润 肿瘤转移 

分 类 号：R737.9[医药卫生—肿瘤]