不同载体材料固定化Fusarium.sp修复老化污染土壤中HMW-PAHs的研究  

Impacts of different immobilization materials for Fusarium. sp on the remediation of aged soils polluted with HMW-PAHs

作　　者：张雪娜[1,2] 贾海滨[1] 王伟[1] 张丽秀[1] 石维[1] 冯圣东[1,2] 杨志新[1,2] ZHANG Xuena JIA Haibin WANG Wei ZHANG Lixiu SHI Wei FENG Shengdong YANG Zhixin(College of Resource and Environment Science, Agricultural University of Hebei, Baoding 071000 Key laboratory for Farm land Eco-environment, Baoding 071000)

机构地区：[1]河北农业大学资源与环境科学学院,保定071000 [2]河北省农田生态环境重点实验室,保定071000

出　　处：《环境科学学报》2017年第3期1107-1114,共8页Acta Scientiae Circumstantiae

基　　金：河北省教育厅项目(No.Z2013058)~~

摘　　要：本研究选用玉米、黄瓜和番茄秸秆作为载体材料,固定化PAHs高效降解菌ZH-H_2(Fusarium.sp),通过土壤培养试验对煤矿区农田高环多环芳烃(HMW-PAHs)污染老化土壤进行修复研究.结果表明,土壤培养60 d后,3种材料固定化ZH-H_2处理(Y+B,H+B,X+B)对10种HMW-PAHs总量均有一定的修复效果,其降解率分别为21.65%、16.10%和15.49%,且Y+B处理的修复效果高于其他两种材料处理(p<0.05);3种载体材料固定化ZH-H_2对土壤HMW-PAHs不同环数的降解存在一定的差异,其对6环PAHs的修复效果均优于4环和5环;3种材料固定化ZH-H_2对Flt、Pyr、Ba A、Chry、Bb F、Ba P 6种单体的降解也存在明显的不同.对Flt、Pyr、Ba A、Chry、Bb F、Ba P的修复效果表现为Y+B处理显著高于H+B和X+B处理,且Y+B处理中尤其对Bb F(24.20%)、In P(24.24%)的修复效果表现突出;对Bghi P单体的修复效果表现为X+B处理显著高于Y+B和H+B,高达30.95%;3种处理对Bk F、Db A和In P 3种PAHs单体的修复效果无差异显著性.进一步分析与HMW-PAHs降解有关的土壤酶活性发现,土壤木质素过氧化物酶和过氧化氢酶活性在3种秸秆材料处理下呈现了较为相似的规律,均表现为Y+B处理下最高,与固定化ZH-H_2对Flt、Pyr、Ba A、Chry、Bb F、Ba P 6种单体PAHs的修复效果规律相一致;而对于多酚氧化酶活性却呈现了相反的规律,表现为Y+B处理下最低的变化特征.综上,对于煤矿区农田污染老化土壤中10种PAHs总量的修复以玉米秸秆作为菌株ZH-H_2的固定化载体材料最为适宜.A soil incubation experiment was carried out to investigate the effect of different carrier materials for Fusarium.sp ZH-H2 immobilization on the remediation of HMW-PAHs in long-term polycyclic aromatic hydrocarbons（ PAHs） polluted soils in coal mining areas. Materials of corn,cucumber,and tomato straws were considered in the present study. After 60-day soil incubation,three carrier materials（ Y＋B,H＋B,X＋B） with immobilized Fusarium.sp played positive roles on the removal of a total amount of 10 HMW-PAHs,where the 10-PAHs removals of treatment Y ＋ B,H ＋ B and X ＋ B were21.65%,16.10% and 15.49%,respectively,and the treatment Y＋B had significantly higher 10-PAHs removal than treatments of H＋B and X＋B（ P〈0.05）. These three materials showed distinctive removal effects on ring-number differed HMW-PAHs,where apparently higher removals of 6-ring PAHsthan 4-ring PAHs and 5-PAHs were observed. Meanwhile,for the six individual HMW-PAHs such as Flt,Pyr,Ba A,Chry,Bb F and Ba P,significantly higher removals by treatment Y＋B in contrast to treatments of H＋B and X＋B were found,especially for Bb F（ 24.20%）,In P（ 24.24%）,respectively. In addition,treatment X＋B showed the highest removal on Bghi P（ 30.95%） in comparison with Y＋B and H＋B,however,little difference in the removals of individual Bk F,Db A and In P among Y＋B,H＋B and X＋B were observed. Further analyses of the soil enzyme activities related to HMW-PAHs removal showed that the enzyme activities of lignin peroxidase and catalase among three treatments showed similar pattern that treatment Y ＋ B had significantly higher enzyme activities than H＋B and X＋B（ P〈0.05）. This was consistent with the removals of Flt,Pyr,Ba A,Chry,Bb F and Ba P. However,treatment Y＋B showed the lowest activity of polyphenol oxidase. In conclusion,corn straw was considered to be a suitable carrier material for Fusarium. sp immobilization to phytoremediate the aged soils contaminated with ∑10PAHs in coal mining areas.

关 键 词：Fusarium.sp 玉米秸秆 黄瓜秸秆 番茄秸秆 HMW-PAHs 

分 类 号：X173[环境科学与工程—环境科学] X53