HPLC法测定菇茑果实中叶黄素的含量  

Determination of Lutein in Physali alkekengi L. by HPLC

作　　者：苟晓亮 刁全平[1] 郭华[1] 刘雍贵[1] 王晓慧[1] Gou Xiaoliang Diao Quanping Guo Hua Liu Yonggui Wang Xiaohui(College of Chemistry and Life Science, Anshan Normal University, Anshan 114007, China)

机构地区：[1]鞍山师范学院化学与生命科学学院,辽宁鞍山114007

出　　处：《山东化工》2017年第5期67-68,共2页Shandong Chemical Industry

基　　金：辽宁省大学生创新创业训练计划项目(2015101690015)

摘　　要：建立用于测定菇茑果实中叶黄素含量的检测方法。方法以正己烷为溶剂,采用浸泡法提取样品中的叶黄素,NaOH-CH_3OH溶液皂化后利用HPLC测定其含量。色谱柱为Kromassil C_(18)柱(5μm,250 mm×4.6 mm),流动相为甲醇,流速为1.0 mL/min,检测波长为445nm。结果叶黄素在2.3~36.8μg/mL范围内呈良好的线性关系。结论该方法准确度高、重现性好,可适于菇茑果实中叶黄素含量的定量分析。To establish the method of determination for Lutein in Physali alkekengi L.. Lutein was extrated by soaking methordwith hexane as solvent, and was aponified by NaOH -CH3OH, and the content was determined by HPLC with methanol asmobile phase at the flow of 1.0 mL/min-l, Kromasil CIS as Column, 445 nm as the detection wavelength. The linear relationshipof Lutein was 2.3-36.8μg/mL. This method was accurate and steady, and can be used for the determination of Lutein inPhysali alkekengi L.

关 键 词：菇茑 叶黄素 HPLC 

分 类 号：O657.7[理学—分析化学]