嗜热复合菌系对不同结构偶氮染料的转化脱色性能  

The Effect of Thermophilic Microflora on Transformation and Decolorization Characteristics of Azo Dyes with Different Structures

作　　者：陈燕[1] 王园秀[1] 肖婷[1] 沈飞[1] 王春莲[1] 张庆华[1] CHEN Yan WANG Yuan-xiu XIAO Ting SHEN Fei WANG Chun-lian ZHANG Qing-hua(Jiangxi Engineering Laboratory of the Development and Utilization of Agricultural Microbial Resources, College of Bioscience and Engineering ,Jiangxi Agricultural University, Nanchang 330045, Chin)

机构地区：[1]江西农业大学生物科学与工程学院/江西省农业微生物资源开发与利用工程实验室,江西南昌330045

出　　处：《江西农业大学学报》2017年第1期153-160,共8页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金(31660024);江西省自然科学基金(20151BAB204001);江西省教育厅科技项目(GJJ14297;GJJ150436);江西农业大学大学生创新训练计划项目(201610410105)~~

摘　　要：用前期筛选出的嗜热复合菌系对天蓝、刚果红、甲基橙、酸性大红、苋菜红等5种偶氮染料进行脱色转化,探究了其对不同结构偶氮染料的脱色转化能力。研究发现,嗜热复合菌系对拥有一个偶氮键且分子量较小的甲基橙和苋菜红展现出良好的脱色效果,在测试浓度范围内(200~1 000 mg/L)几乎不受染料浓度的影响,其脱色率始终维持在99%以上,而其它分子量较大的、含有2个偶氮键的染料(酸性大红、刚果红以及甲基橙)则随着染料浓度的提高,其脱色率则逐渐下降。此外,随着培养时间的延长,嗜热复合菌系对不同偶氮染料的脱色率均呈现逐渐上升的趋势。在前9 h的培养过程中,分子量小结构简单的甲基橙、苋菜红以及酸性大红的脱色率可达到95%以上的较高水平,而分子量大、结构复杂的刚果红和天蓝的脱色率经过13 h的培养其最高脱色率仅可达到84%和73%左右的较低水平。上述结果表明,嗜热复合菌系对不同结构偶氮染料的脱色效果与染料的浓度、分子量、偶氮键的数目以及染料结构的复杂性有关。染料浓度越高、分子量越大、偶氮键数目越多、结构越复杂,复合菌系对染料的转化脱色能力越弱。A previously screened thermophilic microflora was used to investigate the decolorization and transformation effect on five kinds of azo dyes including Sky blue,Congo red,Methyl orange,Acid scarlet and Amaranth,and the decolorization ability on azo dyes with different structures. In this study,it was found that thermophilic microflora owns good decolorization effect on azo dyes of Methyl orange and Amaranth which containing only one azo bond and smaller molecular weights,where the decolorization rates were always above 99%during the test concentration ranges（ 200-1 000 mg / L）,and the decolorization effects were almost not influenced by the concentration of dyes. However,the decoloriztion ratios of the azo dyes containing two azo dye bonds and larger molecular weights（ Acid scarlet,Congo red and Methyl orange） decreased with the increment of the concentrations of azo dye.In addition,the decolorization rates of thermophilic microflora on different azo dyes showed an increasing tendency with the prolongation of the incubation time.During the former 9 h of incubation,the decolorization rates of Methyl orange,Amaranth and Acid scarlet with smaller molecular weights and simple structures could reach high levels of above 99%.However,the highest decolorization rates of Congo red and Sky blue with large molecular weight and complex structure could only reach lower levels of about 84%and 73% respectively after 13 h of incubation.The above results indicated that the decolorization effect of the microflora on different azo dyes were correlated with the concentration,molecular weight,number of azo dye bonds and the structure complexity of azo dyes. Lower decolorization abilities of thermophilic microflora were observed when the azo dyes were of higher concentration,larger molecular weight,larger azo dye bond number and higher structural complexity.

关 键 词：偶氮染料 不同结构 复合菌系 转化脱色 

分 类 号：X172[环境科学与工程—环境科学]