DNA条形码在山茶属近缘植物鉴别中的应用（英文）  

DNA barcodes for identification of closely related species in Camellia

作　　者：温贝贝[1] 邓丽[1] 叶霞[1] 贺巍[1] 刘建军[1] 苏会[1] 李美凤[1] 冯建灿[1] WEN Bei-bei DENG Li YE Xia HE Wei LIU Jian-jun SU Hui LI Mei-feng FENG Jian-can(College of Horticulture, Henan Agricultural University, Zhengzhou 450002, Chin)

机构地区：[1]河南农业大学园艺学院,河南郑州450002

出　　处：《广东农业科学》2017年第1期55-65,共11页Guangdong Agricultural Sciences

基　　金：Science and technology innovation team support plan of Henan university(14IRTSTHN011)

摘　　要：山茶属(Camellia)是山茶科(Theaceae)中物种丰富、经济效益较高的属。分类学上对山茶属的亚属、组以及种的划分争议较大,著名的3大山茶属植物分类系统分别由Sealy、张宏达和闵天禄提出。DNA条形码技术是指通过对植物基因组中的特定基因进行片段扩增、测序发现其碱基变化规律的技术手段,在分类学研究中显示出巨大的应用潜力。选取trn H-psb A和mat K序列的通用引物,对山茶属不同植物基因组DNA进行扩增和序列测定,分别得到了ttrn H-psb A序列和mat K序列各61条,山茶属mat K序列相似度极高(98.40%),物种分辨率较低。trn H-psb A的属间物种分辨能力达到100%,而在山茶属内物种分辨率仅为13.11%。结果表明:trn H-psb A序列能够有效地区分不同属间的植物,但种间分类能力较弱。The Camellia genus containing about 280 species is the only genus with great commercial values in the family Theaceae. The products of this genus such as seed-oil, ornamental plants and different kinds of tea are popular in the current market. The classification of species in Camellia genus using molecular methods may have potential power in finding cryptic species, rearing new variety and controlling the trade involving counterfeit. DNA barcoding is regarded as a promising tool for species-level identification marked by short, standardized DNA sequences. In this study, we tested the value of widely used barcoding markers （ trnH-psbA and matK） on 64 Camellia species and compared with those of other genera in different family downloaded from NCBI. In all, 61 trnH- psbA sequences and 61 matK sequences were obtained and the pairwise distances, aligned length, variable sites were calculated. As the consistency of matK sequences was extremely high （ 98.40% ）, we mainly focused on further analysis of trnH-psbA region. In genus level, all 61 species were successfully distinguished from 7 other genera. When comes to species level within Camellia genus, the discrimination rate was only 13.11%. The results indicate that trnH-psbA region may have effective ability in genus-level identification, but has weak practicability in species- level discrimination.

关 键 词：山茶属 trn H-psb A MAT K DNA条形码 物种分辨率 

分 类 号：S682.31[农业科学—观赏园艺]