田间烟蚜种群对3种杀虫剂抗性与酶活性关系的研究  

Relationship between three insecticides resistance and enzyme activity in natural population of Myzus persicae(Sulzer)

作　　者：张超[1] 刘童童[1,2] 杨洪[1,3] 王召[1,4] 杨茂发[1,3] ZHANG Chaol LIU Tong-tongl＇ YANG Hong WANG Zhaol＇ YANG Mao-fa(Institute of Entomology, Guizhou University, Guiyang 550025, China Guizhou Academy of Forestry Science, Guiyang 550000, China College of Tobacco Science, Guizhou University, Guiyang 550025, China School of Environment and Life Sciences, Kaili College, Kaili 556011, China)

机构地区：[1]贵州大学昆虫研究所,贵州贵阳550025 [2]贵州省林业科学研究院,贵州贵阳550000 [3]贵州大学烟草学院,贵州贵阳550025 [4]凯里学院环境与生命科学学院,贵州凯里556011

出　　处：《广东农业科学》2017年第1期100-105,共6页Guangdong Agricultural Sciences

基　　金：中国烟草总公司贵州省公司科技项目(201022)

摘　　要：为了探讨烟蚜对杀虫剂抗性与酶活关系,就田间烟蚜种群对乐果、灭多威和阿维菌素3种杀虫剂的抗性与酶活性的关系进行研究。对具有不同抗性水平的几个田间烟蚜种群的酶活性测定结果显示:抗乐果、灭多威、阿维菌素品系中AchE、GSTs和CAT 3种酶的活性都高于敏感品系,其中抗乐果的兴仁和湄潭抗性品系AchE酶活性为敏感品系的1.212、1.588倍,GSTs酶活性为敏感品系的1.625、1.900倍,CAT酶活性是敏感品系的1.439、2.018倍;抗灭多威品系的清镇和湄潭抗性品系AchE酶活性为敏感品系的1.078、1.647倍,GSTs酶活性为敏感品系的1.142、1.935倍,CAT酶活性为敏感品系的1.142、1.935倍;抗阿维菌素的兴仁和长顺抗性品系AchE酶活性为敏感品系的1.370、1.664倍,GSTs酶活性为敏感品系的1.830、2.318倍,CAT酶活性为敏感品系的1.800倍、3.708倍。研究结果可为阐明田间烟蚜种群抗性产生机制提供参考。In order to explore the relationship between insecticide resistance and enzyme activity in natural population of Myzus persicae （ Sulzer ） , we studied the activities of Ache, GST and CAT in M. persicae （ Sulzer ） insecticide resistance to dimethoate, methomyl and avermectins. The results showed that, the resistance stains （different regions ） of dimethoate, methomyl and avermectins had higher enzyme activity of AchE, GSTs and CAT than susceptible strain. For the resistance stain of dimethoate, the enzyme activities of AchE in Xingren and Meitan resistant strain were 1.212 times, 1.588 times than susceptible strain; the enzyme activities of GSTs resistant strain were 1.625 times, 1.900 times than susceptible strain, and the enzyme activities of CAT resistant strain were 1.439 times and 2.018 times than susceptible strain. For the resistance strain of methomyl, the enzyme activities of AchE in Qingzhen and Meitan resistant strain were 1.078 times 1.647 times than susceptible strain; the enzyme activities of GSTs resistant strain were 1.142 times, 1.935 times than susceptible strain, and the enzyme activities of CAT resistant strain were 1.142 times, 1.935 times than susceptible strain. Forthe resistance strain of avermectins, the enzyme activities of AchE in Xingren, Changshun resistant strain were 1.370 times, 1.664 times than susceptible strain; the enzyme activities of GSTs resistant strain were 1.830 times, 2.318 times than susceptible strain, and the enzyme activities of CAT resistant strain were 1.800 times, 3.708 times than susceptible strain. The results provide a reference for elucidating the field resistance mechanism of M. persicae （ Sulzer ） population.

关 键 词：烟蚜 抗药性 酶活性 乙酰胆碱酯酶 过氧化氢酶 谷胱甘肽S-转移酶 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]