iRGD-外泌体-阿霉素抑制恶性黑色素瘤体外增殖的研究  

The Inhibition of iRGD-Exosomes-Doxorubicin on the Proliferation of Malignant Melanoma in Vitro

作　　者：赵莉娟[1] 郑江红[1] 柳向东[1] 茅广宇[1] 邓辰亮[1] 杨松林[1] ZHAO Lijuan ZHENG Jianghong LIU Xiangdong MAO Guangyu DENG Chenliang YANG Songlin.(Department of Plastic Surgery, Shanghai Sixth People＇s Hospital, Shanghai Jiaotong University, Shanghai 200233, Chin)

机构地区：[1]上海交通大学附属第六人民医院整形外科,上海市200233

出　　处：《组织工程与重建外科杂志》2017年第1期25-28,共4页Journal of Tissue Engineering and Reconstructive Surgery

摘　　要：目的探讨应用i RGD-外泌体-阿霉素抑制人恶性黑色素瘤细胞系A375体外增殖的有效性及其靶向性。方法体外转染i RGD,并提取外泌体,通过电穿孔包裹阿霉素,利用流式细胞仪检测i RGD-外泌体-阿霉素对A375细胞的亲和力,并对i RGD-外泌体-阿霉素抑制A375细胞的增殖能力进行分析。结果 i RGD-外泌体-阿霉素与A375细胞的结合率高于空白转染-外泌体-阿霉素;i RGD-外泌体-阿霉素可明显抑制A375细胞的增殖,空白转染-外泌体-阿霉素未观察到明显的抑制情况。结论 i RGD-外泌体-阿霉素能通过靶向作用于A375细胞,并抑制其增殖。Objective To investigate the effect and targeting of iRGD-exosomes-doxorubicin （iRGD-exo-dox） on the inhibition of A375 cell line. Methods The exosomes were extracted from the iRGD-transfected A375 cells. The doxorubicin was wrapped by electroporation. Flow cytometry was used to detect the affinity of iRGD-exo-dox to A375 cells. The ability of iRGD-exo-dox on inhibiting the proliferation of A375 cells was analyzed. Results The affinity of iRGD-exo-dox to A375 cells was higher than the blank-exo-dox. The proliferation of A375 cells was obviously inhibited by iRGD-exo-dox. The inhibition effect was not observed in blank-exo-dox group. Conclusion iRGD-exo-dox can inhibit the proliferation of A375 cells by targeting function.

关 键 词：恶性黑色素瘤 靶向治疗 外泌体 阿霉素 

分 类 号：R739.5[医药卫生—肿瘤]