Ras1、Rac1、Rho1基因在阿萨希毛孢子菌酵母相和菌丝相中的表达差异  

Differences in expression of Rasl, Racl and Rhol genes between yeast and hyphal phases of Trichosporon asahii

作　　者：陈珊珊 周剑峰 廖勇 李海涛 王瑞丽 巴根 吕雪莲 杨蓉娅 Chen Shanshan Zhou Jianfeng Liao Yong Li Haitao Wang Ruili Ba Gen Lyu Xuelian Yang Rongya(Department of Dermatology, General Hospital of Beijing Military Command, Beijing 100700, China)

机构地区：[1]解放军陆军总医院皮肤科,北京100700

出　　处：《中华皮肤科杂志》2017年第3期207-210,共4页Chinese Journal of Dermatology

基　　金：国家自然科学基金（81471928、81571972）

摘　　要：目的 研究Ras1、Rac1和Rho1基因在阿萨希毛孢子菌酵母相和菌丝相中的表达差异，探讨其在菌丝生长过程中的作用。方法 分别培养阿萨希毛孢子菌酵母相和菌丝相，提取两组的总RNA，采用荧光定量PCR方法测定两组中Ras1、Rac1和Rho1 mRNA的相对表达量。结果 酵母相菌丝生成率（0.40% ± 0.53%）显著低于菌丝相（99.33% ± 0.57%，t = 13.93，P 〈 0.05）。实时荧光定量PCR显示，以酵母相为对照组，将其Ras1、Rac1、Rho1基因表达量均设为1，则菌丝相的Ras1、Rac1、Rho1基因mRNA相对表达量分别为25.17 ± 10.99、16.81 ± 7.80、42.61 ± 18.50，与酵母相比较，差异有统计学意义（t值分别为3.81、3.51、3.90，均P 〈 0.05）。结论 Ras1、Rac1、Rho1基因可能参与了阿萨希毛孢子菌菌丝的生长过程。Objective To investigate differences in the of Ras1, Rac1 and Rho1 genes between yeast and hyphal phases of Trichosporon asahii （T.asahii）, and to explore their roles in the formation of hyphae. Methods The yeast phase and hyphal phase of T.asahii were cultured and served as yeast phase group and hyphal phase group respectively. Total RNA was extracted from the 2 groups, and real-time fluorescence-based quantitative PCR （RT-PCR） was performed to measure the mRNA of Ras1, Rac1 and Rho1. Results The hyphal formation rate was significantly lower in the yeast phase group than in the hyphal phase group （0.40% ± 0.53% vs. 99.33% ± 0.57%, t = 13.93, P 〈 0.05）. When the mRNA of Ras1, Rac1 and Rho1 in the yeast phase group was all set as 1, that in the hyphal phase group was 25.17 ± 10.99, 16.81 ± 7.80, 42.61 ± 18.50, respectively, with significant differences between the two groups in the three parameters （t = 3.81, 3.51, 3.90, respectively, all P 〈 0.05）. Conclusion Ras1, Rac1 and Rho1 genes may participate in the regulation of hyphal formation in T.asahii.

关 键 词：毛孢子菌属 单体GTP结合蛋白质类 阿萨希毛孢子菌 Rasl RACL Rhol 酵母相 菌丝相 

分 类 号：R379[医药卫生—病原生物学]