EGF, a well-studied mitogen for cancer cells, is revealed to induce an E3 ubiquitin ligase adaptor SPSB1, which recruits the Elongin B/C-Collin complex to trigger ubiquitylation of the negative splicing regulator hnRN...
We thank Drs Xiang-Dong Fu, Zhongsheng You and Min Wei for critical reading of the manuscript and invaluable suggestions, and all the members of Jingyi Hui and Ronggui Hu's laboratories, especially Jiancheng Yu, Xiaojuan Yang and Jie Ye for technical assistance. We are grateful to Dr Xianghui Yu for generously providing us expression plasmids for Elongin B/C, Cu15 and RBX1/2, and to Dr Daming Gao for antilongin B/C antibodies. This work was supported by National Natural Science Foundation of China (31570820, 31661143035 and 31370787) to JH and (31270828, 31470770 and 81525019) to RH and by the National Basic Research Program of China (2012CB910800 and 2013CB910900) to RH. RH was also supported by Shanghai Institute of Organic Chemistry, CAS, and the Instrument Developing Project of CAS (YZ201339).
ExtraceUular signals have been shown to impact on alternative pre-mRNA splicing; however, the molecular mech- anisms and biological significance of signal-induced splicing regulation remain largely unknown. Here, we r...