HK2基因在前列腺癌中的表达及其对前列腺癌细胞恶性表型的影响  

Expression of HK2 in prostate cancer and its effect on malignant phenotype of prostate cancer cells

作　　者：陶陶[1] 沈洲[1] 项平[1] 黄涛[1] 陈恕求[2] 宣强[1] 肖峻[1] 

机构地区：[1]安徽医科大学附属安徽省立医院泌尿外科,合肥230001 [2]东南大学附属中大医院泌尿外科,南京210009

出　　处：《临床与实验病理学杂志》2017年第2期149-152,157,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：安徽省自然科学基金(1708085QH202;1608085MH166)

摘　　要：目的探讨HK2基因在前列腺癌中的表达及其对前列腺癌细胞恶性表型的影响。方法利用基因芯片数据库和免疫组化法检测HK2基因在前列腺癌组织中的表达。应用RNA干扰技术敲低HK2表达后,采用细胞糖代谢试剂盒、CCK-8和流式细胞术检测前列腺癌细胞的有氧糖酵解、增殖和凋亡能力的改变。结果 HK2基因的表达水平随着前列腺癌的进展显著上升;抑制HK2基因表达后,前列腺癌细胞的有氧糖酵解和增殖能力显著降低,细胞的早期凋亡明显增加。结论 HK2基因在前列腺癌的恶性进展中表达上调,促进细胞的增殖和糖代谢,抑制细胞早期凋亡;其潜在分子机制有待进一步研究。Purpose To study the expression of HK2 in human prostate cancer （PCa） tissues and its effect on malignant phenotype of prostate cancer cells. Methods HK2 expression in PCa tissues was determined by microarray database and immunohistochemical staining. Subsequently, the change of cellular phenotype was detected by glycometabolism kit, CCK-8 kit, and flow cytometry after HK2 knockdown. Results HK2 expression was elevated followed by prostate cancer development.HK2 depletion inhibited cellular proliferation and aerobic glycolysis, and increased the ratio of early apoptosis. Conclusion HK2 expression increases in the process of PCa malignant pro- gression. It plays a critical role in cellular proliferation, glycometabolism, and apoptosis, the mechanism of which needs further exploration.

关 键 词：前列腺肿瘤 己糖激酶2 糖代谢 增殖 凋亡 

分 类 号：R737.25[医药卫生—肿瘤]