氟化钠对人牙周膜细胞增殖及矿化能力的影响  

Effect of Na F on proliferation and mineralization of human periodontal ligament cells

作　　者：李坤芩[1,2] 徐乐[1,2] 黄圣运[3] 张东升[1,3] LI kun-qin XU Le HUANG Sheng-yun ZHANG dong-sheng(School of Stomatology of Shandong University. Jinan 250012 Shandong Provincial Key Laboratory of Oral Tissue Regeneration. Jinan 250012 Department of Stomatology, Shandong Provincial Hospital Affiliated to Shandong University. Jinan 250021, Shandong Province, China)

机构地区：[1]山东大学口腔医学院,山东济南250012 [2]山东省口腔组织再生重点实验室,山东济南250012 [3]山东大学附属省立医院口腔科,山东济南250021

出　　处：《上海口腔医学》2017年第1期48-53,共6页Shanghai Journal of Stomatology

摘　　要：目的 :观察氟化钠对体外培养的人牙周膜细胞增殖及矿化能力的影响,为氟添加入牙周组织工程药物中的应用提供依据。方法:原代培养并鉴定人牙周膜细胞,应用CCK8检测不同浓度Na F对h PDLCs增殖的影响,并筛选出4个浓度用于矿化实验。矿化条件下,将0、1×10^(-5)、5×10^(-4)和1×10^(-3) mol/L的Na F作用h PDLCs后,通过碱性磷酸酶(ALP)染色、茜素红染色和实时荧光定量PCR检测矿化能力及成骨相关基因的表达。采用SPSS20.0软件包对数据进行单因素方差分析。结果:5×10^(-5)、1×10^(-4)、5×10^(-4) mol/L的Na F均能促进h PDLCs增殖,且以5×10^(-4) mol/L效果最佳(P<0.05)。而1×10^(-5) mol/L的Na F碱性磷酸酶染色阳性面积最大、茜素红染色矿化结节数量最多(P<0.05)。RT-PCR结果根据时间、指标变化程度较大。结论:5×10^(-5)、1×10^(-4)、5×10^(-4) mol/L的Na F能促进h PDLCs的增殖能力,1×10^(-5) mol/L的Na F能提高h PDLCs的碱性磷酸酶活性及钙结节形成。PURPOSE： To investigate the effect of NaF on proliferation and mineralization of human periodontal ligament cells （hPDLCs）. METHODS： hPDLCs were isolated and characterized. The proliferation of hPDLCs treated with different concentration of NaF was tested by CCK-8. Four moderate concentrations were chosen for subsequent experiments. The mineralization was investigated using ALP activity assay, Alizarin red S staining and quantitative real-time polymerase chain reaction（RT-PCR）. The data were statistically analyzed with SPSS20.0 software package. RESULTS： Immunohistochemistry showed that the isolated cells were hPDLCs. 5×10^-5, 1×10^-4 and 5×10^-4 mol/L NaF had pro-proliferation effects while 5×10^-4 mol/L resulted in the best effect （P〈0.05）. ALP activity and calcium content was significantly enhanced by 1×10^-5 mol/L NaF with osteogenic inductive medium （P〈0.05）. Quantitative RT-PCR data varied in genes as a result of different NaF concentrations and treatment periods. CONCLUSIONS： 5×10^-5, 1×10^-4, 5×10^-4 mol/L NaF can stimulate proliferation in hPDLCs, 1×10^-5 mol/L NaF can enhance ALP activity and calcium content formation of hPDLCs.

关 键 词：氟 牙周膜细胞 矿化 牙周炎 

分 类 号：R781.4[医药卫生—口腔医学]