不同品种金银花精油组分对比研究  

Comparation in essential oil components of different varieties of honeysuckle

作　　者：刘亚[1] 吕兆林[2] 邹小琳[1] 任璇[1] 胡建忠 

机构地区：[1]北京林业大学生物科学与技术学院 [2]北京林业大学分析检测中心 [3]北京市水利部沙棘开发管理中心

出　　处：《北京林业大学学报》2017年第2期72-81,共10页Journal of Beijing Forestry University

基　　金："十二五"国家科技支撑计划项目(2015BAD16B00)

摘　　要：研究同一产地3个不同品种金银花精油的成分,并探索其化合物组成的异同。本实验以3种典型的忍冬科金银花(菰腺忍冬、灰毡毛忍冬、黄褐毛忍冬)为实验材料,采用提取率较高的精油提取装置提取其中的精油,并用气质联用仪(GC-MS)对其进行组分分析。3个不同品种的金银花共检出125种挥发性化合物;通过NIST11标准质谱库检索及人工图谱解析,确定了118种化合物结构。灰毡毛忍冬精油组成以烃类、酯类、醇类为主,其中所占比重最大为醇类,高达71.17%(C10的醇类相对含量为68.93%);化合物种类最多的为C5~C10(27种),占78.28%。菰腺忍冬精油以烃类、醇类为主,其中所占的比重最高为醇类,高达86.3%(C10的醇类相对含量为78.66%);化合物种类最多的为C5~C10(22种),相对百分含量为84.84%;并且没有羧酸类化合物。黄褐毛忍冬精油以酮类、酯类、醇类、醛类为主,其中所占的比例最大的为酯类,高达57.92%(C16~C20的酯类的相对含量为55.61%);种类最多为C5~C10(41种),相对百分含量为34.28%;但是C16~C20的含量最大,为59.33%(种类为15种)。研究表明:金银花种类不同,其精油组成成分在化合物种类、个数及相对含量方面差异很大,通过此次分析为金银花类别的鉴定及其精油的药用价值和香味来源的分析提供数据基础。The composition of essential oil, which was extracted from three different varieties of honeysuckle at the same producing area was studied, and the differences and similarities of the composition of compounds were also explored. This experiment uses three kinds of typical Caprifoliaceae honeysuckle （ Lonicera hypoglauca, Lonicera macranthoides, Lonicera fulvotomentosa ） as experimental material. Meanwhile, the essential oil extraction device with high extraction rate was applied for extracting the essential oil, and combined with the Gas Chromatography-Mass Spectrometer （GC-MS） to assay components. The results showed that 118 components from all of 125 compounds detected in the volatile oil of three varieties of honeysuckle were confirmed according to NISTll library data and manual resolving. Hydrocarbons, esters, alcohols were main compounds in Lonicera macranthoides, and alcohols up to 71.17% was the largest proportion of the total （relative content of C10 alcohols was 68.93% ）. The kind of compound C5 -C10 in Lonicera macranthoides was up to 27 species （78.28%） ; Hydrocarbons, alcohols were the main compounds in Lonicera hypoglauca, and alcohols （86.3%） were the largest proportion of the total （relative content of C^0 alcohols was 78.66% ）. The kind of compound C5 - C^0was up to 22 species （84.84%） , and Lonicera hypoglauca had no carboxylic acid compounds; Lonicera fulvotomentosa mainly contained ketones, esters, alcohols, aldehydes, and the relative content of ester （57.92%） accounted for the largest proportion （relative content of C16 - C20 esters were up to 55.61% ）. C5 -C10 species in Lonicerafulvotomentosa were up to 41 species, whose relative content was 34.28% , but C16 - C20content was the largest, which was up to 59.33% （15 types）. Research had shown that the honeysuckle essential oil components in compound type, number and relative content had great difference because of the variety differences. Moreover, the analysis lays important data base for the classific

关 键 词：金银花精油 气相色谱-质谱联用(GC-MS) 组分对比 

分 类 号：S718.43[农业科学—林学] S789.9