帕金森病大鼠模型尾壳核多巴胺D2受体活性变化研究  

The activity change of dopamine D2 receptors in caudal-putamen of Parkinsons disease model rats

作　　者：张旺明[1] 徐如祥[1] 蔡颖谦[1] 张世忠[1] 杜谋选[1] 

机构地区：[1]第一军医大学珠江医院神经外科,广东广州510282

出　　处：《中华神经外科疾病研究杂志》2002年第3期266-268,共3页Chinese Journal of Neurosurgical Disease Research

基　　金：广东省自然科学基金资助项目[粤科基办2000(25)]

摘　　要：目的 探讨帕金森病病程中多巴胺D2受体的活性变化及其规律。方法 在建立6-羟基多巴胺毁损的帕金森病大鼠模型基础上,应用放射配基结合分析法结合Scanchard作图,测定不同时间点模型大鼠及对照大鼠尾壳核多巴胺D2受体的最大结合容量（Bmax）和平衡解离常数（KD）。结果 大鼠模型毁损侧尾壳核多巴胺D2受体Bmax显著升高,而KD值显著降低,在1个月时达到高峰,受体的亲合力显著增高。结论 帕金森病大鼠模型毁损侧尾壳核存在D2受体上行调节,D2受体明显超敏。Objective To explore the activity change of the dopamine D2 receptor and its rule during the process of Parkinson's disease ( PD ). Methods On the basis of 6-hydroxydopamine-lesioned PD model rats, the radioligand binding assay (RLBA) and Scanchard drawing were used to measure the maximal binding capacity of receptor ( Bmax) and equilibrium dissociation constant ( KD) of the dopamine D2 receptor in the caudal-putamen of the model and control rats on different time-points. Results The RLBA revealed that the dopamine D2 receptor Bmax increased significantly and the KD significantly decreased in the caudal-putamen ipsilateral to the lesion in the model rats, and the changes reached the peak after one month. The D2 receptor affinity increased significantly. Conclusion The study confirms that the dopamine D2 receptors are up-regulated with marked supersensitivity in the caudal-putamen ipsilateral to the lesion in the PD model rats.

关 键 词：帕金森病 多巴胺 多巴胺D2受体 大鼠 放射配基结合分析法 

分 类 号：R742.5[医药卫生—神经病学与精神病学]