抑制Survivin可抑制贝伐珠单抗导致的胶质瘤细胞侵袭性增强  

Knockdown of Survivin expression inhibits glioma cells invasion induced by bevacizumab treatment in glioma cells

作　　者：王鹏[1] 张剑宁[1] 陈金辉[1] 于新[1] 刘锐[1] 孙艳杰[1] WANG Peng ZHANG Jianning CHEN Jinhui YU Xin LIU Rui SUN Yanjie(Department of Neurosurgery , Navy General Hospital, Beijing 100048, China)

机构地区：[1]海军总医院神经外科,北京100048

出　　处：《中华神经外科疾病研究杂志》2017年第1期11-14,共4页Chinese Journal of Neurosurgical Disease Research

摘　　要：目的探讨Survivin对人脑胶质瘤细胞经贝伐珠单抗治疗后侵袭性的影响。方法构建抑制Survivin表达的U251/sur(-)胶质瘤细胞,经贝伐珠单抗处理。采用四甲基偶氮唑蓝法检测细胞增殖,划痕试验检测细胞的迁移能力,Transwell试验检测细胞的侵袭能力。结果经贝伐珠单抗处理后,U251细胞的迁移、侵袭能力明显增强。与亲代U251细胞相比,抑制Survivin的U251/sur(-)细胞经贝伐珠单抗处理后,其迁移和侵袭能力明显降低。与单独抑制Survivin或单独使用贝伐珠单抗相比,将二者合用后胶质瘤细胞的增殖能力明显下降。结论抑制Survivin可明显抑制贝伐珠单抗处理后胶质瘤细胞侵袭性增强,并与贝伐珠单抗产生协同效应,抑制胶质瘤细胞增殖。Objective The study aims to investigate the effect of Survivin on glioma cells invasion after treated by bevacizumab.MethodsSurvivin shRNA plasmids were transfected in to U251 cells. The cells proliferation, migration, and invasion were determined by methyl thiazolyl tetrazolium （MTT） assay, wound healing, and transwell assay, respectively.ResultsThe migration and invasion of U251 cells were increased by bevacizumab. Knockdown of Survivin by RNAi inhibited bevacizumabinduced cell invasion. Furthermore, the combined administration of bevacizumab and knockdown of Survivin significantly suppressed the proliferation of glioma cells compared to bevacizumab treatment or knockdown of Survivin alone.ConclusionDownregulation of Survivin could inhibit glioma cells invasion induced by bevacizumab treatment, therefore Survivin might be a potential target to ameliorate the therapeutic efficiency of bevacizumab.

关 键 词：胶质瘤 贝伐珠单抗 存活蛋白 侵袭 

分 类 号：R739.41[医药卫生—肿瘤]