FISH技术检测TERC基因在CIN等级诊断中的应用  

Application of FISH technology in the detection of TERC gene in CIN grade

作　　者：张晓斌 ZHANG Xiao-bin(Heilongjiang Province Nongken construction of Sanjiang People＇s Hospital, Jiamusi 156300, Heilongjiang)

机构地区：[1]黑龙江省农垦建三江人民医院,黑龙江佳木斯156300

出　　处：《安徽卫生职业技术学院学报》2017年第1期117-118,共2页Journal of Anhui Health Vocational & Technical College

摘　　要：目的:研究FISH技术检测TERC基因在子宫颈上皮内瘤变1级与2级鉴别诊断中的价值。方法:选取50例宫颈组织标本为研究对象,均采用FISH技术检测,分析TERC基因的扩增情况。结果:在正常的子宫颈鳞状上皮中,TERC基因没有扩增,20例初步诊断为2级的患者中,有8例TERC基因扩增,10例初步诊断为1级的患者中,有1例TERC基因扩增,差异存在统计学意义(P<0.05)。结论:采用石蜡包埋组织切片难以对CIN1/2级进行鉴别时,要采用FISH技术检测,如果TERC基因扩增,则判定为CIN2级;TERC基因不扩增,则判定为CIN1级。Objective：To detect the TERC of FISH gene in cervical intraepithelial neoplasia grade 1 and grade 2 diagnostic value.Methods：50 cases of cervical tissue specimens as the research object, were detected by FISH analysis, amplification of TERC gene.Results：in normal cervix squamous epithelium,TERC gene showed no amplification phenomenon, 20 eases of initial diagnosis of 2 patients,8 eases of TERC gene amplification in 10 cases of primary diagnosis of 1 patients, 1 cases of TERC gene amplification,there were statistical significance （P〈 0.05）. Conclusion：using paraffin embedded tissue sections of CIN1/2 are difficult to diagnosis and differential diagnosis of the case,to use the FISH technology to detect if the TERC gene amplification is determined for CIN2;TERC gene amplification,is determined to CIN1 level.

关 键 词：FISH技术 TERC基因 子宫颈上皮内瘤变 鉴别诊断 

分 类 号：R737.33[医药卫生—肿瘤]