血管内皮祖细胞对共植入缺血再灌注模型大鼠神经干细胞增殖、凋亡和血管重建的影响  

Co-culture with vascular endothelial progenitor cells: effects on proliferation and apoptosis of neural stem cells and vascular remodeling in rats with ischemia reperfusion injury

作　　者：杨纯生[1] 贺丹[1] 谭军[1] Yang Chun-sheng He Dan Tan Jun(Department of Neurology, Third Affiliated Hospital of Xinxiang Medical University, Xinxiang 453003, Henan Province, Chin)

机构地区：[1]军新乡医学院第三附属医院神经内科,河南省新乡市453003

出　　处：《中国组织工程研究》2017年第5期718-723,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:神经干细胞移植在缺血性脑血管疾病治疗中的作用已经明确,但细胞不能在移植局部存活等问题限制了进一步的应用。目的:探讨血管内皮祖细胞对共植入缺血再灌注模型大鼠神经干细胞增殖、凋亡和血管重建的影响。方法:125只SD大鼠随机分为5组,假手术组、缺血对照组、神经干细胞组、神经干细胞+血管内皮祖细胞组、血管内皮祖细胞组,每组25只。线栓法制备缺血再灌注大鼠模型,并进行分组干预。移植后1,2,3,4,5周,检测各组神经干细胞增殖、凋亡以及缺血区血管新生情况。结果与结论:(1)移植后不同时间点,血管内皮祖细胞组、缺血对照组、假手术组均未观察到BrdU阳性细胞。神经干细胞+血管内皮祖细胞组的Brd U阳性细胞数显著高于神经干细胞(P<0.05);(2)移植后不同时间点,神经干细胞+血管内皮祖细胞组和神经干细胞组可观察到BrdU+/Caspase-3+双标阳性细胞。神经干细胞+血管内皮祖细胞组的细胞凋亡率显著低于神经干细胞组(P<0.05);(3)移植后不同时间点,假手术组均未见新生血管,其他4组均出现新生血管。神经干细胞+血管内皮祖细胞组的血管新生数量显著多于其他各组(P<0.05);(4)结果表明,血管内皮祖细胞可以促进共植入缺血再灌注模型大鼠缺血半暗带的神经干细胞增殖,抑制细胞凋亡,并促进缺血区血管新生。BACKGROUND： Neural stem cell（NSC） transplantation is a common method for various ischemicencephalopathies, but inability to survive in the transplantation region limits its further use in clinical practice. OBJECTIVE： To explore the effect of vascular endothelial progenitor cells（VEPCs） on the proliferation and apoptosis of co-cultured NSCs as well as vascular remodeling in rats with ischemia reperfusion injury. METHODS： 125 Sprague-Dawley rats were randomly divided into five groups, 25 rats in each group, including sham operation, ischemia, NSCs, co-culture, and VEPCs groups. Rat models of ischemia reperfusion injury were made in all groups except for the sham operation group, followed by corresponding interventions. The proliferation and apoptosis of neural stem cells were detected, and vascular remolding in the ischemic region was observed in each group. RESULTS AND CONCLUSION： At different time points after transplantation, BrdU positive cells were not observed in VEPCs, ischemia and sham operation groups; the number of BrdU positive cells in the co-culture group was significantly higher than that in the NSCs group（P〈0.05）; BrdU ＋/Caspase-3＋ cell were observed in both co-culture and NSCs groups, and the apoptosis rate of the co-culture group was significantly lower than that in the NSCs group（P〈0.05）; there were new blood vessels in all the groups except for the sham operation group, and the number of new bone vessels was highest in the co-culture group. To conclude, our experimental results show that VEPCs promotes the proliferation of co-cultured NSCs, inhibits cell apoptosis and and promote angiogenesis in the ischemic penumbra of rats with ischemia reperfusion injury.

关 键 词：再灌注损伤 神经干细胞 细胞增殖 细胞凋亡 新生血管化 生理性 组织工程 干细胞 移植 缺血性脑病 缺血再灌注 血管内皮祖细胞 干细胞移植 血管新生 

分 类 号：R394.2[医药卫生—医学遗传学]