丙酮酸羧化酶在结直肠癌中的表达及其生物学意义  

Expression and clinical significance of pyruvate carboxylase in colorectal cancer

作　　者：马素珍[1] 刘丹丹[1] 潘晓丽[1] 朱艳琴[1] 

机构地区：[1]河南中医药大学基础医学院实验教学中心,河南郑州450008

出　　处：《暨南大学学报（自然科学与医学版）》2017年第1期79-85,共7页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：河南省高等学校重点科研项目(16A310021)

摘　　要：目的:检测丙酮酸羧化酶(pyruvate carboxylase,PC)在结直肠癌及相应癌旁组织中的表达,并分析PC在结直肠癌发生及进展过程中可能的生物学意义.方法:首先采用免疫组织化学方法测定PC在结直肠癌及相应癌旁组织(距离肿瘤边缘5cm)中的表达水平;SPSS软件统计分析PC蛋白表达水平与结直肠癌患者年龄、性别、肿瘤浸润深度、淋巴结转移、Dukes’分期及病理分化程度等临床病理指标之间的关系,同时分析PC蛋白表达水平与患者生存预后的关系;此外,利用R2数据库http://hgserver1.amc.nl/cgi-bin/r2/main.cgi中的大数据分析PCmRNA基因表达水平对结直肠癌患者预后的影响.结果:PC在结直肠癌组织中的表达水平高于其对应的癌旁组织(P<0.01);而且PC高表达与肿瘤浸润深度、病理分化较差及患者临床分期较高有关(P值均<0.05);此外,PC的表达与患者生存预后显著相关,PC高表达的患者预后较差(P<0.001).结论:PC在结直肠癌组织中高表达,PC与结直肠癌的发生及进展密切相关,是结直肠癌患者独立的预后因素,因此PC可作为治疗结直肠癌的潜在临床靶点.Aim：To study the clinical significance and biological function of pyruvate carboxylase（PC） expression in colorectal cancer. Methods： Immunohistochemical staining were utilized to detect the expression level of PC in colorectal cancer and matched adjacent non-tumor tissues,and the correlations of PC expression with clinicopathological features were studied using statistical analyses. Kaplan-Meier analysis and Cox proportional hazard modeling were used to assess overall survival to analyze the effect of PC expression on prognosis of colorectal cancer. In addition,R2 microarray analysis in http：//hgserver1.amc.nl/cgi-bin/r2/main.cgi was used to analyze the effect of PC mRNA expression on prognosis of colorectal cancer.Results： The PC expression were significantly higher in colorectal cancer than in matched adjacent non-tumor tissues（ P〈0. 05）. PC expression correlated significantly with Dukes’ clinical stage and pathological differentiation degree（ all P 〈 0. 05）. In addition,Patients with high PC levels showed poor survival（ P〈0. 001）.Conclusion：PC expression correlated with aggressive clinical features in color-ectal cancer. And PC might be a potential target for colorectal cancer biotherapy.

关 键 词：丙酮酸羧化酶 结直肠癌 临床病理指标 生存预后 

分 类 号：R735.3[医药卫生—肿瘤]