里氏木霉和黑曲霉产酸性木聚糖酶及酶学特性比较  

Comparative Analysis of the Characterization of Crude Acidic Xylanases from two Strains between Trichoderma reesei and Aspergillus niger

作　　者：吴仁智[1,2] 黄俊[1] 芦志龙[1,2] 陈英[1,2] 陆琦[1] 陈小玲[1] 陈东[1,2] 黄日波[1,2] 

机构地区：[1]广西科学院非粮生物质酶解国家重点实验室国家非粮生物质能源工程技术研究中心广西生物质产业化工程院广西生物炼制重点实验室,广西南宁530007 [2]广西大学生命科学与技术学院,广西南宁530004

出　　处：《广西科学》2017年第1期112-119,共8页Guangxi Sciences

基　　金：国家"863"项目(2013AA050701);国家国际合作项目(2011DFA61910);国家科技计划课题子任务(2012AA022106);广西科技合作与交流计划(桂科合2015DD23055);广西重点研发计划(桂科AB16380024);广西科技攻关/主席科技资金(1517-07);广西科学院基本科研业务费项目(12YJ25SW04);八桂学者建设工程专项经费项目;广西生物质产业化工程院建设(桂科能12237022);广西生物炼制重点实验室项目(13-051-08)资助

摘　　要：【目的】对黑曲霉和里氏木霉产酸性木聚糖酶的性能及所产粗酶的酶学特性进行分析比较,尤其是考察pH值为4时木聚糖酶酶活力及稳定性,从而确定潜在的较为理想的酸性木聚糖酶。【方法】将里氏木霉和黑曲霉接种至培养基进行产酶培养,比较分析两者的酸性木聚糖酶、酸性木糖苷酶的酶活力及酶学特性。【结果】黑曲霉酸性木聚糖酶和酸性木糖苷酶的酶活力最高分别达(52.36±2.61)U/mL和(0.57±0.01)U/mL,酸性木聚糖酶最适温度和pH值分别为55℃、5.0,酸性木糖苷酶最适温度和pH值分别为75℃、5.0;里氏木霉酸性木聚糖酶和酸性木糖苷酶的酶活力最高分别达(10.12±0.95)U/mL和(0.32±0.05)U/mL,酸性木聚糖酶最适温度和pH值分别为65℃、6.5,酸性木糖苷酶最适温度和pH值分别为65℃、4.5。黑曲霉和里氏木霉的酸性木聚糖酶兼有酸性CMCase酶活力,分别为(5.26±0.21)U/mL、(1.72±0.21)U/mL。【结论】黑曲霉所产酸性木聚糖酶明显比里氏木霉的更优良,是潜在的较为理想的酸性木聚糖酶。【Objective】To identify potential ideal acidic xylanase,the characterizations of crude acidic xylanase from two strains between Trichoderma reesei and Aspergillus niger are analyzed and compared,especially when the xylanase activity and stability are determined at pH4.【Methods】These two strains mentioned above are inoculated into culture medium for xylanase production,and the enzyme activity and characterizations of crude acid xylanase are analyzed.【Results】The activities of acidic xylanase and xylosidase fromAspergillus nigerare up to（52.36±2.61）U/mL and（0.57±0.01）U/mL respectively,and the optimum temperature and pH value of acidic xylanase from this strain are 55℃ and 5.0,while the optimum temperature and pH value of acidic xylosidase from this strain are 75℃ and 5.0,respectively.With constract to Trichoderma reesei,they are（10.12±0.95）U/mL and（0.32±0.05）U/mL,65℃ and 6.5,65℃ and 4.5,correspondingly.In addition,both of the acidic xylanases produced by Aspergillus niger and Trichoderma reesei have acidic CMCase activity,of which Aspergillus nigeris（5.26±0.21）U/mL and Trichoderma reesei is（1.72±0.21）U/mL,respectively.【Conclusion】The acidic xylanase produced by Aspergillus niger is potential ideal acidic xylanase,which is better than the one produced by Trichoderma reesei.

关 键 词：黑曲霉 里氏木霉 酸性木聚糖酶 酶学特性 

分 类 号：Q93[生物学—微生物学]