人参皂苷下调IL-1受体相关激酶1基因表达抑制肝癌细胞增殖及促进其凋亡作用及机制  

The effects of Ginsenoside on the proliferation and apoptosis of liver cancer cells by down regulating the Iinterleukin-1 receptor-associated 1 and the potential mechanism

作　　者：王娜[1,2] 刘刚[1,2] 刘欢[1,2] 王策[1,2] 张德新[1,2] 

机构地区：[1]第四军医大学,陕西西安710032 [2]西京消化病医院,陕西西安710032

出　　处：《现代肿瘤医学》2017年第8期1183-1187,共5页Journal of Modern Oncology

基　　金：国家自然科学基金面上项目(编号:81472779)

摘　　要：目的:探索人参皂苷F11对肝脏恶性肿瘤细胞增殖及凋亡的影响。方法:选择四种癌细胞系Hep G2、Hep3B、SMMC-7721、MHCC-97H利用Western blot技术检测IRAK1在这些细胞中的基础表达量。然后选择一种IRAK1高表达的肝癌细胞系对其进行人参皂苷处理以观察该药物对IRAK1分子的影响,分别利用WST-1法检测肿瘤细胞的增殖及流式技术检测细胞凋亡是否发生改变。接着在Hep G2细胞中转染siIRAK1来从RNA及蛋白水平降低该分子后检测肿瘤细胞的增殖。结果:使用人参皂苷或者si IRAK1处理Hep G2细胞可以明显降低IRAK1分子的表达,且它们对肿瘤细胞增殖的抑制率分别是49.6%(P<0.000 1)和45.3%(P<0.05)。未进行人参皂苷处理的Hep G2的凋亡率为2.9%左右,加入10μg/ml的人参皂苷后可以使肝癌细胞的凋亡率增加3倍左右达到12.0%左右(P<0.01)。结论:人参皂苷可以通过降低IRAK1及其活化后的p-IRAK1分子降低肝癌细胞的增殖及促进其凋亡,从而抑制肿瘤的恶行进展。Objective： To explore the effect of Ginsenoside F11 on the proliferation and apoptosis of liver cancer cells.Methods： Firstly,Western blot was used to examine the expressed level of IRAK1 in HepG2,Hep3 B,SMMC-7721,MHCC-97 H cell lines.Then one kind of liver cancer cell line with high expression of IRAK1 were treated with Ginsenoside to observe the growth rates and apoptosis of these cells by using WST-1 and Cytometry methods respectively.Finally,HepG2 cells were transfected with negative control or siIRAK1 which could reduce IRAK1 both in RNA and protein levels,after that we analyzed the proliferation of them.Results： The expression of IRAK1 could be decreased by using Ginsenoside or siIRAK1,as the results of which the cell growth inhibition ratio were 49.6%（P〈0.0100 1） and 45.3%（p〈0.05）.The apoptosis of HepG2 cells increased from 2.9% to 12.0% by adding Ginseniside into the medium of the cell.Conclusion： Ginsenoside could inhibit the malignant progress of liver cancer by suppressing the proliferation and increasing the apoptosis,and this phenomenon is closely related to the reduced IRAK1 and p-IRAK1.

关 键 词：IRAK1 人参皂苷F11 肝癌 增殖 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]