饥饿对硫自养反硝化反应器生物群落结构的影响  

Impact of Starvation Conditions on Biological Community Structure in Sulfur Autotrophic Denitrification Reactor

作　　者：李芳芳[1,2] 施春红[1,2] 李海波[1] 袁蓉芳[1] 马方曙[1] LI Fang-fang SHI Chun-hong LI Hai-bo YUAN Rong-fang MA Fang-shu(College of Energy and Environmental Engineering, Beijing University of Science and Technology, Beijing 100083, China Beijing Key Laboratory of Resource-Oriented Treatment of Industrial Pollutants, Beijing 100083, China)

机构地区：[1]北京科技大学能源与环境工程学院,北京100083 [2]工业典型污染物废物资源化处理北京市重点实验室,北京100083

出　　处：《环境科学》2017年第3期1109-1115,共7页Environmental Science

基　　金：国家水体污染控制与治理科技重大专项(2015ZX07203-011)

摘　　要：采用颗粒硫磺/白云石和黄铁矿/白云石自养反硝化反应器处理二沉尾水,探究饥饿忍耐对反应器去除效果、微生物群落变化的影响及其性能恢复时间.结果表明,在12~14℃的低温条件下,经过30 d的不进水饥饿忍耐后,颗粒硫磺/白云石和黄铁矿/白云石反应器出水NO_3^--N浓度分别从1.78 mg·L^(-1)、11.32 mg·L^(-1)增加到27.87 mg·L^(-1)、26.56 mg·L^(-1).颗粒硫磺/白云石和黄铁矿/白云石自养反硝化反应器分别在重启后的第5 d和11 d使得NO_3^--N去除性能恢复至饥饿前的水平,并且在低温条件下保持了良好的脱氮效果.MiSeq高通量测序结果表明,2个反应器的细菌群落的丰度和多样性饥饿期均低于恢复期,且优势菌门均为Proteobacteria,主要的纲类为β-Proteobacteria.硫磺/白云石反应器中鉴别出Thiobacillus菌属为主要反硝化类群.Sulfur / dolomite and pyrite / dolomite autotrophic denitrification reactors were applied to treat the secondary effluent of wastewater treatment plant to explore the removal effect,the changes of microbial community,and recovery time of reactors after starvation period. It was shown in the results that after 30 d non-water starvation endurance,the effluent concentrations of NO_3--N in sulfur / dolomite and pyrite / dolomite reactors increased from 1. 78 mg·L（-1）,11. 32 mg·L（-1）to 27. 87 mg·L（-1）,26. 56 mg·L（-1）respectively at the low temperature of 12-14℃. In addition,sulfur / dolomite and pyrite / dolomite reactors recovered within 5 d and 11 d since restarted and could maintain a good effect of nitrogen removal at low temperature. Mi Seq high throughput sequencing results showed that the abundance and diversity of the bacterial communities in starvation period in both reactors were lower than those in recovery period. The dominating phylum was Proteobacteria in both reactors while the dominating class was β-Proteobacteria.Thiobacillus was identified as the main genus for denitrification in sulfur / dolomite reactor.

关 键 词：硫自养反硝化 饥饿期 低温 MiSeq高通量测序 微生物多样性 群落结构 

分 类 号：X703.1[环境科学与工程—环境工程]