微小RNA-148a抑制乳腺癌细胞MDA-MB-231迁移的作用机制  

The mechanisms of miR-148a inhibiting migration of breast cancer MDA-MB-231 cells

作　　者：韩丽[1] 江茜[1] 何苗[1] 颜媛媛[1] 刘一诺[1] 魏敏杰[1] 

机构地区：[1]中国医科大学药学院药理教研室,辽宁省分子靶向抗肿瘤药物药理学研究与评价重点实验室,辽宁沈阳110122

出　　处：《现代肿瘤医学》2017年第7期997-1001,共5页Journal of Modern Oncology

基　　金：国家自然科学基金项目(编号:81572898);辽宁省高校创新团队计划资助项目(编号:LT2014016);辽宁省科技计划项目(编号:2014226033);辽宁省教育厅科学研究一般项目(编号:L2015596);沈阳市科技计划项目(编号:F16-094-1-00)

摘　　要：目的:研究微小RNA-148a(miR-148a)在乳腺癌细胞株MDA-MB-231中的表达,探讨上调miR-148a的表达对其迁移的影响及机制。方法:采用实时荧光定量PCR(qRT-PCR)检测正常乳腺上皮细胞MCF-10A和乳腺癌细胞株MDA-MB-231中miR-148a的表达水平。应用脂质体法将miR-148a mimic及阴性对照分别转染MDA-MB-231细胞,qRT-PCR检测转染效率;细胞划痕实验检测转染前后MDAMB-231细胞迁移能力的变化;Western blot方法检测上皮间质转化(EMT)相关蛋白Slug、Snail和E-cadherin表达水平的变化。结果:与MCF-10A细胞相比,MDA-MB-231细胞中miR-148a的表达水平明显降低(P<0.05);与阴性对照组相比,miR-148a转染组中miR-148a的表达水平显著升高(P<0.05);过表达miR-148a后,MDA-MB-231细胞迁移能力明显下降,Slug和Snail蛋白表达明显降低,E-cadherin蛋白表达明显增加。结论:miR-148a可通过调控Slug、Snail及E-cadherin蛋白的表达抑制EMT,进而抑制乳腺癌细胞的迁移能力。Objective：To study the expression of miR- 148 a in MDA- MB- 231 breast cancer cells,and to explore the effect and mechanism of miR- 148 a on the breast cancer cells migration. Methods：Quantitative real- time PCR（ qRT- PCR） was used to detect the expression of miR- 148 a in MCF- 10 A cells and human breast cancer cell line MDA- MB- 231. miR- 148 a mimic and negative control were transfected into MDA- MB- 231 cells. The efficiency of transfection was measured by qRT- PCR. Cell scratch assay was used to observe the migration ability changes. Western blot was performed to detect the expression levels of epithelial- mesenchymal transition（ EMT） related proteins Slug,Snail and E- cadherin. Results：The expression level of miR- 148 a in MDA- MB- 231 cells decreased significantly（ P 0. 05）. miR- 148 a mimic obviously increased the expression of miR- 148 a,inhibited the cell migration,decreased Slug and Snail proteins expression,increased E- cadherin protein expression in MDA- MB- 231 cells（ P 0. 05）. Conclusion：miR- 148 a may inhibit EMT and migration by regulating the expression of Slug,Snail and E- cadherin of breast cancer cells.

关 键 词：乳腺癌 miR-148a 迁移 上皮间质转化 

分 类 号：R737.9[医药卫生—肿瘤]