miR-124抑制多形性胶质母细胞瘤的生长  

miR-124 inhibits the growth of glioblastoma

作　　者：吕中华[1] 谭春雷[1] 王洪滨[1] 耿建雄 常亮[1] 苏君[1] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院神经外科,黑龙江哈尔滨150040

出　　处：《现代肿瘤医学》2017年第7期1027-1029,共3页Journal of Modern Oncology

基　　金：黑龙江省青年科学基金(编号:QC2013C093)

摘　　要：目的:研究下调miR-124和过表达miR-124对恶性胶质瘤细胞增殖的影响,在分子病因学方面阐述miR-124在调控胶质瘤细胞的生长中起到的重要作用。方法:在胶质瘤细胞系中应用RT-PCR定量方法检测下调miR-124来评价miR-124的作用。应用病毒转染的过表达miR-124的稳定细胞系U87-124和U373-124,用qRT-PCR方法检测过表达miR-124对胶质瘤细胞增殖的影响。结果:miR-124在4个胶质瘤细胞系(U87、U373、SW1088和SW1073)中与对照组细胞系相比呈明显低表达水平。U87-miR-124和U373-miR-124的胶质瘤细胞增殖明显低于对照组。结论:在分子病因学方面,miR-124能明显抑制脑胶质瘤细胞的增殖,在调控脑胶质瘤细胞的生长中起到了重要作用,同时也为miR-124用于脑胶质瘤的治疗提供了一个可能性。Objective：To study the effects of downgrade of miR- 124 and the overexpression of miR- 124 for malignant glioma cell proliferation,the effects of the molecular etiology in the regulation of miR- 124 for the growth of glioma cells. Methods：Quantitative RT- PCR method was used in glioma cell line cut of miR- 124 test to evaluate the role of miR- 124. Application of virus transfection of the stability of the overexpression of miR- 124 cell line in U373- 124 and U87- 124,qRT- PCR was used to detect the expression of miR- 124 effects on glioma cell proliferation. Results：miR- 124 in all four glioma cell lines（ U87,U373,SW1088 and SW1073） compared with control group in cell lines had significantly lower expression. Glioma cell proliferation in U87- miR- 124 and U373- miR-124 were significantly lower than the control group. Conclusion：In terms of molecular etiology,miR- 124 can significantly inhibit the proliferation of glioma cells,in regulating the growth of glioma cells,as well as providing a possibility for the treatment of glioma.

关 键 词：miR-124 多形性胶质母细胞瘤 抑制 

分 类 号：R730.264[医药卫生—肿瘤]