2013年-2015年舟山海岛地区人乳头状瘤病毒E6/E7基因变迁分析  

Analysis of HPV E6/E7 gene trend in Zhoushan from 2013 to 2015

作　　者：周晓 杜玲 ZHOU Xiao DU Ling(Zhoushan Maternal and Child Health Care Hospital, Zhoushan, Zhejiang 316004, China)

机构地区：[1]舟山市妇幼保健院,浙江舟山316004

出　　处：《中国卫生检验杂志》2017年第4期588-589,593,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的调查和分析2013年-2015年舟山地区妇女的人乳头状瘤病毒(HPV)E6/E7 mRNA阳性率变迁。方法选取2013年1月-2015年12月在本院就诊的本地常住妇女503例,采集宫颈脱落细胞,荧光定量PCR法检测HPV E6/E7 mRNA表达,以HPV E6/E7 mRNA>103为阳性。结果共计HPV E6/E7 mRNA阳性88例,总阳性率为17.49%。≤30岁组中,2015年的HPV E6/E7 mRNA阳性率高于2014年,差异有统计学意义(χ~2=4.08,P<0.05),≥41岁组中,2015年的HPV E6/E7 mRNA阳性率高于2014年,差异有统计学意义(χ~2=8.92,P<0.01)。2015年的乡村组HPV E6/E7 mRNA阳性率高于2014年,差异有统计学意义(χ~2=11.14,P<0.01)。结论舟山地区≥41岁和乡村妇女是HPV E6/E7 mRNA阳性高发人群,有必要对其加强宫颈癌病变监控。Objective To investigate the trend of human papillomavirus（HPV） E6/E7 gene in women from Zhoushan.Methods 503 local clinical women were selected for the collection of cervical cells from January 2013 to December 2015.HPV E6/E7 gene expression was detected by quantitative real-time PCR.Higher than 103 copies of mRNA expression was set as positive.Results Total positive rate of HPV E6/E7 gene expression was 17.49%（88/503）.In ≤30 age group,the positive rate of HPV E6/E7 gene in 2015 was significantly higher than 2014,with the differences statistically significant（χ^2= 4.08,P〈0.05）.In ≥41 age group,positive rate of HPV E6/E7 gene in 2015 was significantly higher than 2014,with the differences statistically significant（χ^2= 8.92,P〈0.01）.In village group,positive rate of HPV E6/E7 gene in 2015 was significantly higher than 2014,with the differences statistically significant（χ^2= 11.14,P〈0.01）.Conclusion Rural women ≥ 40 in Zhoushan is a high incidence group of HPV mRNA E6/E7,and it is necessary to strengthen the monitoring of cervical cancer lesions.

关 键 词：人乳头状瘤病毒 荧光定量聚合酶链式反应 E6/E7基因 

分 类 号：R737.33[医药卫生—肿瘤]