油水淹地石油降解菌群的筛选和鉴定及高效菌群的构建  

Screening and Identification of Microorganism Producing Biosurfactant from Oil-water Flooded Soil and Construction of High Efficient Microbiome Colony

作　　者：王铁媛[1] 窦森[1] 胡永哲[1] 林琛茗 WANG Tieyuan DOU Sen HU Yongzhe LIN Chenming(College of Resources and Environment, Jilin Agricultural University, Changchun 130118, China)

机构地区：[1]吉林农业大学资源与环境学院,长春130118

出　　处：《吉林农业大学学报》2016年第6期716-722,共7页Journal of Jilin Agricultural University

基　　金：国家"973"项目(2011CB10053);吉林省科技发展计划项目(20120405)

摘　　要：从油水淹地污染土壤中获得26株石油降解菌,筛选出表面活性剂产生菌1株(H-6)和优势菌6株(H-1、H-17、H-18、H-19、H-20、H-23),结合菌株形态观察、革兰氏染色和16S r DNA序列同源性分析,鉴定表面活性剂产生菌H-6为堀越氏芽孢杆菌(Bacillus horikoshii)。以表面活性剂产生菌H-6为中心,再任选3株优势菌株构建石油降解菌群,得到高效石油降解菌群C5(H-1、H-6、H-18、H-19),通过正交试验得到菌群C5各菌种的最佳接种量。结果表明:接种量15%(H-1)、15%(H-6)、20%(H-18)、10%(H-19),温度25℃,石油含量2 000 mg/L条件下,7 d时石油降解率达到78.87%。这说明高效降解菌群C5对石油具有较好的降解效果,可应用于油水淹地污染土壤的修复。One microorganism producing biosurfacant （H-6） and 6 dominant strains （ H- 1, H- 17, H-18, H-19,H-20 and H-23） were screened from 26 strains of oil degradation bacteria obtained from oil-water flooded and contaminated soil. In combination with strain morphological observation, gram staining and 16S rDNA sequence homology analysis, microorganism producing biosurfactant H -6 was identified as Bacillus horikoshii. With H-6 as the center, 3 dominant strains were randomly selected to construct oil degradation microbiome colony, high efficient oil degradation microbiome C5 （H-l, H-6, H-18, H-19） was obtained, and the best inoculum sizes of strains in the microbiome C5 were defined by orthogonal test. The results show that 7 d oil degradation rate achieves 78.87% under the conditions of inoculation size 15% （ H- 1 ）, 19）, temperature 25 ℃, and oil content 2 000 mg/L. 15% （H-6）, 20% （H-18） Therefore, high efficient degradati 10% （H- on microbiome C5 has good effect of oil degradation, and can be applied to repair oil-water flooded and contaminated soil.

关 键 词：油水淹地 表面活性剂产生菌 高效石油降解菌群 堀越氏芽孢杆菌 

分 类 号：X131.3[环境科学与工程—环境科学]