喷雾干燥法制备饲用植酸酶制剂  

Preparation of Feed Phytase by Spray-drying

作　　者：张琪[1] 朱庆锋[1] 崔百元[1] 孔谦[1] 王志林[2] 晏石娟[1] ZHANG Oi ZHU Qingfeng CUI Baiyuan KONG Qian WANG Zhilin YAN Shijuan(Agro-biological Gene Research Center, GDAAS , Guangzhou 510640, China Institute of Animal Science, Guangdong Academy of Agricultural Sciences, State Key Laboratory of Livestock and Poultry Breeding, Guangzhou 510640, China)

机构地区：[1]广东省农业科学院农业生物基因研究中心,广州510640 [2]广东省农业科学院动物科学研究所,畜禽育种国家重点实验室,广州510640

出　　处：《吉林农业大学学报》2016年第6期750-753,共4页Journal of Jilin Agricultural University

基　　金：广东省农业攻关引导项目(2012B020305011);广东省科技计划项目(2011A020102009,2013A061401020);广东省农业领域科技计划项目(2013B020305005)

摘　　要：以重组植酸酶(phyA-ABCD5F7)的毕赤酵母工程菌为植酸酶生产菌,经高密度发酵制备得到粗酶液。通过在粗酶液中添加Mg^(2+)和Zn^(2+)等金属离子以及海藻糖,可明显提高植酸酶的热耐受能力。以植酸酶的酶活回收率为评价指标,考察了进风温度、出风温度和保护剂对植酸酶酶活回收率的影响,确定最佳喷雾条件:保护剂5 g/L MgSO_4·7H_2O、2 g/L ZnSO_4·7H_2O和10 g/L海藻糖,进风温度为160℃,出风温度为70~75℃,在此条件下,植酸酶的酶活回收率达80%。Recombinant pichia pastoris can secrete engineered phytase （phyA-ABCD5F7）. After its high-density fermentation, crude enzyme liquid was prepared. Heat tolerance of phyA-ABCD5F7 can be improved via adding trehalose and metal ions such as Mg^2＋and Zn^2＋. The effects of inlet/out- let air temperature and several protectors were evaluated, according to their effects upon phytase ac- tivity recovery after spray-drying. The optimum spray-drying conditions were determined： 5 g/L Mg- SO4 · 7H=O, 2 g/L ZnSO4· 7H2O and 10 g/L trehalose added as protectors, and 160℃/70 ～75℃ for inlet /outlet air temperature. Under such conditions, the recovery of phytase activity reached 80%.

关 键 词：植酸酶 喷雾干燥 保护剂 酶活 回收率 

分 类 号：Q814.4[生物学—生物工程]