GenomeLab-(TM) GeXP检测rhIL-24联合顺铂对肺癌细胞凋亡相关基因表达的影响  

GenomeLab^(TM) GeXP analysis system to detect apoptosis-associated genes in A549 cells treated by recombinant human IL-24 combined with cisplatin

作　　者：郭锦锦[1] 王少慧[1] 孙万邦[1] 唐艳丽[1] 姜广斌[1] 宋明英[1] 

机构地区：[1]遵义医学院珠海校区,贵州省免疫学研究生教育创新基地,广东珠海519041

出　　处：《细胞与分子免疫学杂志》2017年第1期44-47,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：遵义医学院硕士启动基金项目(F-719)

摘　　要：目的探讨重组人白细胞介素24(rhIL-24)联合顺铂(DDP)诱导人肺腺癌A549细胞凋亡相关基因的变化。方法用160 ng/m L rhIL-24、3μg/m L DDP及160 ng/m L rhIL-24联合3μg/m L DDP处理A549细胞,应用Genome LabTMGe XP多基因遗传表达分析系统检测凋亡相关基因Bax、Bcl-2、存活蛋白(survivin)、caspase-3、视网膜母细胞瘤基因(Rb)、p53的mRNA表达水平。结果 rhIL-24蛋白处理的A549细胞上调Bax、caspase-3、Rb的mRNA水平,下调Bcl-2、survivin的mRNA水平,但p53mRNA变化无规律,rhIL-24联合DDP组对A549细胞上述基因表达的影响更明显。结论 rhIL-24蛋白可通过上调Bax、抑癌基因Rb,下调Bcl-2、survivin,激活caspase-3而诱导人肺腺癌A549细胞凋亡。Objective To investigate the changes of apoptosis-associated genes in human lung adenocarcinoma cell line A549 after treated by the recombinant human interleukin-24（ rhIL-24） combined with cisplatin（ DDP）. Methods The mRNA levels of Bax,Bcl-2,survivin,caspase-3,retinoblastoma（ Rb） gene,p53 were analyzed by Genome LabTMGe XP genetic analysis system after A549 cells were treated with 160 ng/m L rhIL-24 or 3 μg/m L DDP or 160 ng/m L rhIL-24 combined with3 μg/m L DDP. Results The rhIL-24 up-regulated the levels of Bax,Rb,caspase-3 mRNAs and down-regulated Bcl-2,survivin mRNAs,but the change of p53 mRNA was not regular. The combination of rhIL-24 and DDP strengthened the above changes. Conclusion The rhIL-24 protein can induce the apoptosis of A549 cells by up-regulating Bax,caspase-3,Rb mRNAs and down-regulating Bcl-2,survivin mRNAs.

关 键 词：GenomeLabTM GeXP多基因遗传表达分析系统 白细胞介素24(IL-24) 肺癌细胞 细胞凋亡 

分 类 号：R734.2[医药卫生—肿瘤]