黄芪多糖逆转EC109/DDP食管癌细胞顺铂耐药的可能机制  

Mechanism of astragalus polysaccharide's reversing effect on EC109/DDP esophageal cancer cell's resistance to cisplatin

作　　者：刘晓滨[1] 祁丽[1] 张文轩[1] 

机构地区：[1]哈尔滨医科大学附属第二医院肿瘤放疗科,哈尔滨1500860

出　　处：《癌症进展》2017年第1期29-31,共3页Oncology Progress

摘　　要：目的研究黄芪多糖逆转EC109/DDP食管癌细胞顺铂耐药的可能机制。方法将EC109/DDP顺铂耐药食管癌细胞分为CON组(未行任何药物干预)、APS组(仅黄芪多糖干预)、DDP组(仅顺铂干预)及APD组(黄芪多糖及顺铂干预),比较4组细胞吸光度、细胞周期、凋亡率及MRP和GST-π基因表达的差异。结果 APD组EC109/DDP顺铂耐药食管癌细胞的D1~D6吸光度、S期、G_2/M期和增殖指数均低于CON组、APS组和DDP组(P﹤0.05),G_0/G_1期和凋亡率均高于CON组、APS组和DDP组(P﹤0.05)。CON组和DDP组间、APS组和APD组间EC109/DDP顺铂耐药食管癌细胞MRP和GST-π基因m RNA表达差异均无统计学意义(P﹥0.05);APS组和APD组细胞MRP和GST-π基因m RNA表达均低于CON组和DDP组(P﹤0.05)。结论黄芪多糖可逆转EC109/DDP顺铂耐药食管癌细胞对顺铂的耐药性,其可能机制与黄芪多糖显著降低MRP和GST-π基因表达有关。Objective To study the mechanism of Astragalus polysaccharide’s reversing effect on Ec109/DDP esophagus cancer cell’s resistance to Cisplatin. Methods Ec109/DDP esophagus cancer cells were divided into CON group （no astragalus polysaccharide and Cisplatin treatment）, APS group （only astragalus polysaccharide treatment）, DDP group （only Cisplatin treatment） and APD group （Astragalus polysaccharide and Cisplatin treatment） according to their treatment methods. The differences of cell absorption, cell cycle, apoptosis and expressions of MRP and GST-π were compared. Results The D1-D6 absorption, S stage, G2/M stage and PI in APD group were significantly lower than in CON group, APS group and DDP group （All P〈0.05）, but G0/G1 stage and cell apoptosis rate were higher （All P〈0.05）. The differences of MRP and GST-π mRNA expressions were not statistics significant between APS group and APD group, but the were lower than in CON group and DDP group （All P〈0.05）. Conclusion Astragalus polysaccharide could significantly reverse Ec109/DDP esophagus cancer cell’s resistance to Cisplatin, its mechanism may be related with lowering down MRP and GST-π’s expression.

关 键 词：食管癌 黄芪多糖 顺铂 耐药 机制 

分 类 号：R735.1[医药卫生—肿瘤]