枯草芽孢杆菌工程菌发酵技术的研究进展  

Research progress of the fermentation technology of genetic engineering bacteria of Bacillus subtilis

作　　者：黄福佳[1] 姜宁[1] 张爱忠[1] 全佳慧 张晨雪[1] 孙海森[1] 杨萧华 申林用 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《黑龙江畜牧兽医》2017年第3期63-67,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31472120);黑龙江省自然科学基金项目(C201325);黑龙江省农垦总局科技攻关项目(HNK125B-12-04);黑龙江省大学生创新创业训练计划项目(201510223035);大庆市科技攻关项目(szdfy-2015-20);黑龙江八一农垦大学科研培育重点项目;黑龙江八一农垦大学研究生创新科研项目(YJSCX2015-Y24)

摘　　要：枯草芽孢杆菌既是有益菌,又是表达外源蛋白的工具,其产生的工程菌的许多特性随着其所携带的基因发生一定变化。工程菌生物发酵的目的是扩大携带外源基因的菌体分泌蛋白量和菌体自身的产量。文章主要介绍枯草芽孢杆菌的表达系统,分析发酵条件控制和补料策略对基因工程菌体生产量的影响,并对枯草芽孢杆菌工程菌发酵领域的研究现状进行综述。Bacillus subtilis is both a beneficial bacterium and a tool for expressing heterologous proteins, and many properties of the engineering bacteria produced by Bacillus subtilis are changed with its carried gene. The purpose of biological fermentation of engineering bacteria is to enlarge the amount of secreted protein of bacteria carrying exogenous gene and the yield of bacteria themselves. This paper mainly introduces the expression system of Bacillus subtilis, and analyzes the effects of fermentation condition control and feeding strategies on the production of genetic engineering bacteria, and also reviews the research status in the fermentation field of genetic engineering bacteria of Bacillus subtilis.

关 键 词：枯草芽孢杆菌 基因工程菌 表达系统 发酵 条件控制 补料策略 

分 类 号：S852.616[农业科学—基础兽医学]