苹果抗炭疽菌叶枯病基因SNP和InDel标记的HRM筛选  

Screening of SNP and InDel Markers to Glomerella Leaf Spot Resistance Gene Locus in Apple Using HRM Technology

作　　者：刘源霞[1] 兰进好[2] 柏素花[3] 孙晓红[3] 刘春晓[1] 张玉刚[1] 戴洪义[1] 

机构地区：[1]青岛农业大学园艺学院青岛市园艺植物遗传改良与育种重点实验室,山东青岛266109 [2]青岛农业大学农学与植物保护学院,山东青岛266109 [3]青岛农业大学生命科学学院,山东青岛266109

出　　处：《园艺学报》2017年第2期215-222,共8页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31372032);国家现代农业产业技术体系建设专项资金项目(CARS-28-01-07);山东省良种产业化工程项目(620902);"泰山学者"建设工程和青岛市民生科技计划项目(15-9-2-99-nsh;14-2-3-38-nsh);‘十二五’国家科技支撑计划项目(2013BAD02B01);山东省优秀中青年科学家科研奖励基金项目(BS2014SW027)

摘　　要：以207株‘金冠’与‘富士’苹果的F1杂交分离群体实生树为试材,挑选抗炭疽菌叶枯病基因R_(gls)位点区域内的,第15条染色体上,位于SSR标记S0304673和S0405127之间500 kb物理距离内,由全基因组重测序技术所获得的与抗该病相关的10个SNP及10个InDel标记,通过高分辨率熔解曲线(High Resolution Melting,HRM)技术筛选出与基因R_(gls)位点紧密连锁的2个SNP及4个InDel标记。通过对重组个体的基因型及表型分析,发现标记InDel4227和InDel4254表现出与R_(gls)基因位点共分离,将基因R_(gls)精细定位于标记InDel4199和SNP4299之间100 kb的物理距离内。对该基因位点两侧4.1~4.3Mb距离内的基因进行统计分析,表明在该区段内存在40个有功能注释的基因,涉及到细胞组成、分子功能和生物过程方面共19个GO分类。In present study,10 SNP and 10 InDel markers associated with Glomerella leaf spot（GLS）resistance in the region of Rgls,on apple linkage group（LG）15,in a region of 500 kb between S0304673 and S0405127 markers,obtained by using the whole genome sequencing technology were tested in the population of 207 F1 individuals derived from a cross between‘Golden Delicious’ and‘Fuji’apples by High Resolution Melting（HRM）. Two SNP and 4 InDel markers tightly linked to the Rgls locus were screened. Through analyzing the genotypes and phenotypes of the recombinant individuals,the Rgls locus was fine mapped within a distance of 100 kb between InDel4199 and SNP4299 markers on the‘Golden Delicious’ genome sequence and was co-segregated with InDel4227 and InDel4254 markers. Forty genes were predicted within the region of 4.1–4.3 Mb flanked the Rgls locus,including cellular component,molecular function and biological process in a total of 19 GO classifications.

关 键 词：苹果 高分辨率熔解曲线 炭疽菌叶枯病 SNP标记 INDEL标记 精细定位 

分 类 号：S661.1[农业科学—果树学]