海洋枝顶孢霉BH0531发酵液杀线虫组分的分离及活性测定  

Separation and activity determination of nematicidal components from marine-derived Acremonium sp.BH0531 fermentation broth

作　　者：刘晓霞[1] 李娜[1] 苗丽华[1] 申佩娟[1] 冯欣[1,2] 孟庆恒[1,2] 孙建华[1,2] 

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]天津市动植物抗性重点实验室,天津300387

出　　处：《中国酿造》2017年第3期35-38,共4页China Brewing

基　　金：国家自然科学基金资助项目(31272019);天津市自然基金联合项目(15JCYBJC51400)

摘　　要：实验采用生物活性跟踪法,通过超滤、Sephadex凝胶层析、离子交换树脂、硅胶板薄层层析,以及特异性鉴别染色等方法对海洋枝顶孢霉BH0531发酵液的杀线虫活性组分进行分级分离和初步的性质分析。结果表明,Sephadex凝胶柱层析(G-25,G-10)可有效分离获得杀线虫活性组分,3 d的线虫校正死亡率达98.33%;以正丁醇:水:无水乙醇=1∶1∶1(V/V)为展开剂的薄层层析,可将活性组分有效分离,R_f=0.31,斑点洗脱物的线虫校正死亡率达到94.00%;定性染色结果显示,茚三酮反应阳性,证实有α-氨基存在,茚三酮吡啶呈红色,显现出寡肽性质;双甲酮染色呈亮黄色,表明具有酮糖性质。结果表明,BH0531杀线虫活性代谢物应为含有酮糖基团的短肽类化合物。The nematicidal active components from marine-derived Acremonium sp. BH0531 fermentation broth were fractionated and preliminarily identified through the methods of ultra-filtration, Sephadex gel chromatography, anion-exchange resin, thin layer chromatography （TLC）, as well as specific differential staining under the guidance of bio-active assay. The results showed that the nematicidal active components were separated and purified effectively by Sephadex G-25 and Sephadex G-10 gel chromatography, the adjusted mortality of the nematodes at 3 d was up to 98.33%. The TLC results indicated that the active components can be effectively separated using 1-butanol, H20, anhydrous ethanol （1：1：1） （V/V） as developing solvent, Rf= 0.31. The spot of silica gel plate, which adjusted mortality of the nematodes was 94.00%. The qualitative staining results indicated that the inhydrin color reaction was positive, which proved the presence of α-amino group. The inhydrin pyridine color reaction was red, showing oligopeptide properties. The dimedone staining was bright yellow, showing the properties of ketose. These results suggested that the marine-derived fimgus Acremonium sp. BH0531 fermentation broth with nematicidal activity should contain oligopeptides compounds with ketose groups.

关 键 词：海洋枝顶孢霉 杀线活性组分 活性跟踪 分离 

分 类 号：Q939.9[生物学—微生物学]