猪线粒体抗病毒信号蛋白MAVS对口蹄疫病毒感染宿主的影响  

MAVS with porcine mitochondrial antiviral signaling protein on host cell infected by FMD virus

作　　者：崔晓蕊[1,2] 李守湖 秦晓东[2] 常兴妮 孙鹏[2] 孙继国[1] 李志勇[2] 

机构地区：[1]河北农业大学动物医学院,河北保定071001 [2]中国农业科学院兰州兽医研究所,甘肃兰州730046 [3]甘肃农业大学动物医学院,甘肃兰州730070

出　　处：《中国兽医学报》2017年第3期393-397,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31201943);国家国际科技合作专项资助项目(2011DFA31830);国家"863"计划资助项目(2011AA10A211-3)

摘　　要：采用构建猪源MAVS重组质粒pEGFP-MAVS,将其转染PK-15细胞24h后,再感染口蹄疫病毒(MOI=0.1),通过Western blot和real-time PCR检测MAVS融合蛋白表达及对口蹄疫病毒感染宿主细胞的复制和病毒感染滴度。结果显示:重组质粒pEGFP-MAVS能在PK-15细胞中获得表达,并且上调MAVS基因对口蹄疫病毒在宿主细胞中的早期复制有显著抑制作用,病毒的感染滴度也有一定的降低。结果表明:MAVS具有一定的抗病毒作用,此为进一步研究口蹄疫病毒逃逸宿主天然免疫的机制奠定了基础。To study the impact of swine mitochondrial antiviral signaling protein MAVS on the in- fection of foot-and-mouth disease virus （FMDV） to host cells,recombinant plasmid swine MAVS pEGFP-MAVS was constructed, after transfecting PK-15 cells 24 h, and then infected FMDV （MOI=0. 1）. Western blot and real-time PCR were used to detect expression of MAVS fusion protein and its replication of FMDV infection to host cell and virus titer. The results showed that the recombinant plasmid pEGFP-MAVS could be expressed in PK-15 cells. And the upregulation MAVS gene can significant inhibition the host cell early replication,and there was a little decrease in virus titer. The study showed that MAVS has some antiviral function,which provided basis for further research of FMDV escape mechanisms to host innate immune.

关 键 词：线粒体 MAVS 口蹄疫病毒 实时荧光定量PCR 

分 类 号：S855.3[农业科学—临床兽医学]