瘦素促进人乳腺癌细胞株MCF-7增殖的机制  

The effects of Leptin in proliferation of MCF-7 breast cancer cells

作　　者：刘文慧[1] 张秋瑾[1] 周心怡[1] 张杰[1] 骆耐香[1] LIU Wenhui ZHANG Qiujin ZHOU Xinyi ZHANG fie LUO Naixiang(Department of Immunology, Guilin Medical University, Guilin 541004, China)

机构地区：[1]桂林医学院免疫教研室,541004

出　　处：《实用医学杂志》2017年第4期514-517,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:81260317)

摘　　要：目的:探讨瘦素是否通过影响Rab-25和C-myc的表达促进人乳腺癌细胞株MCF-7细胞的增殖。方法:体外培养人乳腺癌MCF-7细胞株,在对数生长期将细胞随机分为对照组和瘦素处理组,一定时间后MTT法检测细胞增殖程度以及Rab-25和C-myc的表达水平。结果:与对照组相比,不同浓度瘦素处理MCF-7细胞,一定范围内可呈时间和剂量依赖性的方式促进细胞增殖(P<0.05)。瘦素处理组MCF-7细胞Rab-25和C-myc蛋白表达水平均高于对照组(P<0.05),差异有统计学意义;Rab-25 m RNA的表达水平瘦素处理组高于对照组(P=0.05),差异无统计学意义;而C-myc m RNA的表达水平瘦素组高于对照组(P<0.05),差异有统计学意义。结论:瘦素通过上调Rab-25和C-myc特别是后者的表达水平促进乳腺癌MCF-7细胞增殖,为临床乳腺癌的靶向基因治疗提供新的实验依据。Objective To investigate the effects of leptin on proliferation of the breast carcinoma MCF-7 cells in terms of protein and mRNA of Rab-25 and C-myc. Methods MCF-7 cells were treated with leptin and normal saline （NS） as control. The 490 mn OD values of cells were read by MTT and the protein and mRNA expressions of Rab-25 and C-myc were measured by western blot and RT-PCR methods in two groups. Results Compared with control group, leptin can promote the proliferation of MCF- 7 cells in a dose and time dependent manner within certain limit and up-regulate the protein expressions of Rab-25 and C-myc in the breast carcinoma MCF-7 cells （P 〈 0.05）. However, leptin also can up-regulate the mRNA expressions of Rab-25 but without significance （P = 0.05）. On the contrary, leptin can also up-regulate the mRNA expressions of C-myc （P 〈 0.05）. Conclusion Leptin promotes breast cancer cells proliferation, which may be related to up-regulate the protein and mRNA expressions of Rab-25 and C-myc. It could provide theoretical foundation and new target of gene therapy in the clinical treatment of breast cancer.

关 键 词：乳腺肿瘤 瘦素 Rab-25 C-MYC 增殖 

分 类 号：R737.9[医药卫生—肿瘤]