不同化学物质对银耳孢子细胞膜通透性影响  

Effects of different chemical substances on the permeability of spore membrane of Tremella fuciformis

作　　者：艾柳英 翁彩红 阮玲云[1] 张海洋[1] 赖春芬 陈文星[1] 孙淑静[1] 

机构地区：[1]福建农林大学生命科学学院,福建福州350002 [2]中国石油大学(华东)化学工程学院,山东青岛266555

出　　处：《菌物学报》2017年第3期339-348,共10页Mycosystema

基　　金：国家自然基金青年项目(31201669);教育部博士点基金(20103515120015);福建省自然基金杰出青年项目(2014J06010);财政部;农业部重大农技推广项目(KNJ-153033)~~

摘　　要：为了提高外源基因表达胞外分泌水平,本研究通过测定不同化学物质对银耳孢子转化子细胞生物量、培养液电导率及胞外漆酶酶活的影响,探索不同化学物质对银耳孢子细胞膜通透性的影响。结果表明,浓度分别为1%、0.3%、0.2%和0.1%的二甲基亚砜、triton X‐100、氯化钾、两性霉素B均可显著提高电导率;同时银耳孢子胞外漆酶酶活分别提高至7.00U/L、6.67U/L、5.00U/L和5.00U/L,与对照达到显著差异。而表面活性剂(SDS)及有机溶剂(甲苯、戊二醛)则不利于银耳孢子的生长及漆酶的分泌。该研究结果显示,添加一定的化学物质对促进目标产物的外泌有较为明显的作用,这将为银耳孢子作为生物反应器高效表达外源基因提供科学依据。The effect of different chemical substances on the permeability of cell membrane of Tremefa fuciformis spore was nvestigated in detail by analyzing the effect of different chemical substances on the cell biomass of T. fuciformis transformant, and conductivity and extracellular laccase production of fermentation broth. The results showed that the conductivity could be reproved when T..fuciformis spore was treated with 2% dimethyl sulfoxide （DMSO）, 0.3% tritonX-200, 0.2% potassium chloride and 0.1% amphotericin B, respectively, and the activity of extracellular laccase produced by 7：. J：uciformis spore can reach 7.00U/L,6.67U/L, 5.00U/L and 5.00U/L, respectively. As compared with the control, the difference the high foaming surfactant （sodium dodecyl sulfate, SDS） and organic solvents （toluene, the cell growth and laccase secretion of T. fuciformis spore. These results show that the enhance the extracellular excretion of target products and the current work forms the efficient expression of exogenous gene using T..fuciformis spore as bioreactor. was statistically significant. However, glutaraldehyde） were unfavorable for addition of chemical substances can basis for further study on the high

关 键 词：转化子 化学物质 细胞生物量 电导率 胞外漆酶酶活 

分 类 号：S567.34[农业科学—中草药栽培]