接种伯克氏菌D54对东南景天吸收富集Cd的影响  

The effect of inoculating Burkholderia sp. D54 on cadmium uptake and accumulation in Sedum alfredii Hance

作　　者：张亚经 马成仓[1] 郭军康[3] 韩磊[2] 冯人伟[2] 冯雪敏[2] 曹雲清 王瑞刚[2] 

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]农业部环境保护科研监测所,天津300191 [3]陕西科技大学环境科学与工程学院,西安710021 [4]天津农学院农学与资源环境学院,天津300384

出　　处：《农业环境科学学报》2017年第3期449-457,共9页Journal of Agro-Environment Science

基　　金：国家自然科学基金项目(41541026,41373128)~~

摘　　要：通过水培盆栽试验,利用非损伤微测等技术手段,研究了接种伯克氏菌D54对超积累生态型东南景天(HE)和非超积累生态型东南景天(NHE)不同组织中Cd、Zn、Fe、Mg和Mn含量、根尖Cd^(2+)动态吸收以及Cd的转移系数的影响。结果发现,HE茎、叶中Cd、Zn含量,茎/根、叶/根Cd的转移系数以及根尖不同部位Cd^(2+)内流速率均显著高于NHE。Cd处理显著增加了HE和NHE根、茎、叶中Fe和Zn的含量,以及茎中Mg的含量;另外,Cd处理显著降低了HE根、茎、叶中Mn的含量,但却显著增加了NHE根和茎中Mn的含量。伯克氏菌D54处理显著降低了HE和NHE茎、叶中Cd、Fe、Mg、Mn和Zn的含量,茎/根、叶/根Cd的转移系数以及根尖不同部位Cd^(2+)的内流速率。这表明HE对Cd的转移和富集能力显著高于NHE,且HE对Cd的超富集能力与根部对Cd的强吸收能力密切相关。Cd处理促进了HE和NHE对Fe、Zn和Mg的富集,抑制了HE对Mn的富集和促进了NHE对Mn的富集,可能与HE超富集Cd和Mn产生竞争有关。伯克氏菌D54能减少HE和NHE对Cd的吸收和富集,同时导致Fe、Mg、Mn和Zn的富集减少。A water culture experiment was carried out to investigate the effect of inoculating Burkholderia sp. D54 on Cd, Zn, Fe, Mg and Mn content in different organ, Cd2＋ dynamic absorption of root tips, and Cd transfer factor（TF） in hyperaccumulating ecotype of Sedum alfredii（ HE ） and non hyperaccumulating ecotype of Sedum alfredii（ NHE ） using a non-invasive micro-test technique etc. Results showed that the Cd and Zn contents in the stems and leaves, the Cd TF of stem/root and leaf/root, the Cd：＋ influx rate in different locations of roots in HE were significantly higher than those in NHE. The addition of Cd increased the content of Fe and Zn in roots, stems and leaves of HE and NHE, as well as the content of Mg in the stems of HE and NHE. In addition, the addition of Cd significantly reduced the content of Mn in the roots, stems and leaves of HE, but significantly increased the content of Mn in roots and stems of NHE. Inoculating Burkholderia sp. D54 significantly reduced the content of Cd, Fe, Mg, Mn and Zn in the stems and leaves, the Cd TF of stem/root and leaf/root, the Cd2＋ influx ratein different locations of roots in HE and NHE. This indicated that the ability of HE to transport and accumulate Cd was significantly higher than that of NHE, which was closely related to the stronger ability to absorb Cd in the roots of HE. The addition of Cd promoted the accumulation of Fe, Mg and Zn in HE and NHE. However, the addition of Cd inhibited the Mn accumulation in HE but enhanced that in NHE, which may be related to Cd and Mn competition in HE. Inoculating Burkholderia sp. D54 reduced the uptake and accumulation of Cd, Fe, Mg, Mn and Zn.

关 键 词：东南景天 镉 伯克氏菌 吸收 富集 

分 类 号：X171.5[环境科学与工程—环境科学]