石灰与黑麦草对Cu污染土壤的修复及对微生物群落的影响  

Remediation of Cu contaminated soil and microbial community as affected by combining lime with ryegrass

作　　者：杜志敏[1,2] 郭雪白 王继雯[1,2] 岳丹丹[1,2] 周静[4] 巩涛[1,2] 杨文玲[1,2] 甄静[1,2] 慕琦[1] 陈国参[1,2] 

机构地区：[1]河南省科学院生物研究所有限责任公司,郑州450008 [2]河南省微生物工程重点实验室,郑州450008 [3]郑州工商学院,郑州450000 [4]中国科学院南京土壤研究所,南京210008

出　　处：《农业环境科学学报》2017年第3期515-521,共7页Journal of Agro-Environment Science

基　　金：2017年河南省科学院基本科研业务费专项(铜尾矿耐性菌株的筛选及其对黑麦草生长影响研究);2016年河南省科学院基本科研业务费专项(石灰对铜污染土壤微生物群落的影响研究);2012年河南省重大科技专项(121100110100);2014年河南省转制机构研究发展专项[豫财政(2014)383号]~~

摘　　要：通过盆栽试验研究了Cu污染土壤中添加不同剂量石灰(质量分数分别为0.1%、0.2%和0.4%,编号SH1、SH2和SH3)对黑麦草(Secale cereale)生长及Cu吸收、土壤Cu形态转化、土壤微生物群落变化等的影响,以此评价石灰对Cu污染土壤的修复效果。试验结果表明,石灰处理后黑麦草生物量及Cu吸收量均显著增加,黑麦草地上部及根系Cu含量均显著降低;SH1、SH2和SH3处理与对照相比,土壤pH显著升高,土壤可交换态Cu含量分别显著降低了58.47%、87.51%和74.54%,而碳酸盐结合态Cu含量显著增加,分别达到对照处理的1.36、1.93、1.56倍。Biolog测试结果表明,培养72 h时,石灰处理后土壤微生物AWCD值、基质丰富度S、Shannon、Simpson和Mc Intosh指数均有不同程度增加,表现为SH2>SH3>SH1>CK;石灰处理后土壤微生物群落的碳源利用模式发生明显变化,SH2处理可利用的碳源种类最多,碳源利用程度最高。研究表明,施用质量分数0.2%石灰并种植黑麦草对该Cu污染土壤具有较好修复效果。A pot experiment was set up to test the remediation effects in a Cu contaminated soil by applying of lime（SH1, SH2 and SH3, dosages of 0.1%, 0.2% and 0.4%, respectively） and ryegrass（Secale cereale ）, according to the determination of growth and Cu accumula- tion of ryegrass, transformation of Cu speciation in soil, and soil microbial community. The results showed that lime significantly increased biomass and Cu accumulation of ryegrass and soil pH, but decreased Cu concentration of aboveground and root of ryegrass. Compared to control, SH1, SH2 and SH3 decreased exchangeable Cu by 58.47%, 87.51% and 74.54%, while increased carbonate bound Cu to 1.36, 1.93 and 1.56 times than control, respectively. The Biolog analysis at 72 h showed that lime had the ability to increase AWCD （average well color development）, S（substrate richness）, Shannon＇s, Simpson, and McIntosh diversity index in the descending order of SH2, SH3, SH1 and CK. Lime changed the carbon source utilization patterns of soil microbial communities obviously. SH2 had the highest level of the car- bon source utilization. In conclusion, combining lime of dosage of 0.2% with ryegrass could remediate the Cu contaminated soil.

关 键 词：石灰 黑麦草 修复 CU 污染 BIOLOG 

分 类 号：X53[环境科学与工程—环境工程]